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CD56 ELISA 试剂盒

NCAM1 适用: 小鼠 AA 20-711 Colorimetric Sandwich ELISA 625-40.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1889387
发货至: 中国
  • 抗原 See all CD56 (NCAM1) ELISA试剂盒
    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
    抗原表位
    AA 20-711
    适用
    • 8
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    625-40.000 pg/mL
    最低检测浓度
    625 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD56/NCAM1
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: L20-T711
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <20pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: L20-T711
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 2 fibronectin type-III domains.

    板类型
    Pre-coated
    实验流程
    mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.281, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 17.2, Standard deviation: 0.946, CV(%): 5.5
    • Sample 3: n=16, Mean(ng/ml): 28.6, Standard deviation: 1.83, CV(%): 6.4,
    • Sample 1: n=24, Mean(ng/ml): 8.3, Standard deviation: 3.984, CV(%): 4.8
    • Sample 2: n=24, Mean(ng/ml): 19.5, Standard deviation: 1.326, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 32.5, Standard deviation: 2.34, CV(%): 7.2
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CD56 (NCAM1) ELISA试剂盒
    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
    别名
    NCAM1 (NCAM1 产品)
    别名
    CD56 ELISA Kit, MSK39 ELISA Kit, NCAM ELISA Kit, fj56g09 ELISA Kit, ncam ELISA Kit, ncam1 ELISA Kit, wu:fj56g09 ELISA Kit, NCAM1 ELISA Kit, n-cam ELISA Kit, E-NCAM ELISA Kit, NCAM-1 ELISA Kit, Ncam ELISA Kit, Cd56 ELISA Kit, N-CAM ELISA Kit, N-CAM-1 ELISA Kit, NCAM-C ELISA Kit, NCAMC ELISA Kit, N-CAM-1-B ELISA Kit, NCAM-1-B ELISA Kit, ncam1-a ELISA Kit, ncam1-b ELISA Kit, neural cell adhesion molecule 1 ELISA Kit, neural cell adhesion molecule 1a ELISA Kit, neural cell adhesion molecule 1 S homeolog ELISA Kit, neural cell adhesion molecule 1 L homeolog ELISA Kit, NCAM1 ELISA Kit, ncam1a ELISA Kit, ncam1 ELISA Kit, LOC100085373 ELISA Kit, ncam1.S ELISA Kit, Ncam1 ELISA Kit, ncam1.L ELISA Kit
    背景

    Protein Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

    Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellâ€"cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

    Synonyms: Neural cell adhesion molecule 1,N-CAM-1,NCAM-1,CD56,Ncam1,Ncam,

    Full Gene Name: Neural cell adhesion molecule 1

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    基因ID
    17967
    UniProt
    P13595
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