MSMB ELISA 试剂盒
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- 抗原 See all MSMB ELISA试剂盒
- MSMB (Microseminoprotein, beta (MSMB))
- 抗原表位
- AA 21-114
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PSP94
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S21-I114 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S21-I114 - Featured
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Microseminoprotein, beta (MSMB) ELISA Kit
MSMB 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Seminal Plasma, Serum, Tissue Homogenate
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenates
Microseminoprotein, beta (MSMB) ELISA KitMSMB 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the beta-microseminoprotein family.
Tissue Specificity: Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder. .
- 板类型
- Pre-coated
- 实验流程
- human PSP94 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PSP94 has been precoated onto 96-well plates. Standards(E.coli, S21-I114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PSP94 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PSP94 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human PSP94 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PSP94 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 765, Standard deviation: 36.72, CV(%): 4.8
- Sample 2: n=16, Mean(pg/ml): 1533, Standard deviation: 78.2, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 2897, Standard deviation: 182.5, CV(%): 6.3,
- Sample 1: n=24, Mean(pg/ml): 890, Standard deviation: 56.96, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 1915, Standard deviation: 137.88, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 3143, Standard deviation: 238.9, CV(%): 7.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all MSMB ELISA试剂盒
- MSMB (Microseminoprotein, beta (MSMB))
- 别名
- MSMB (MSMB 产品)
- 别名
- HPC13 ELISA Kit, IGBF ELISA Kit, MSP ELISA Kit, MSPB ELISA Kit, PN44 ELISA Kit, PRPS ELISA Kit, PSP ELISA Kit, PSP-94 ELISA Kit, PSP57 ELISA Kit, PSP94 ELISA Kit, MSMB ELISA Kit, PIP ELISA Kit, beta-MSP ELISA Kit, microseminoprotein beta ELISA Kit, microseminoprotein, beta ELISA Kit, beta-microseminoprotein ELISA Kit, microseminoprotein, beta- ELISA Kit, MSMB ELISA Kit, Msmb ELISA Kit
- 背景
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Background: Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory(tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.
Synonyms: Beta-microseminoprotein,Immunoglobulin-binding factor,IGBF,PN44,Prostate secreted seminal plasma protein,Prostate secretory protein of 94 amino acids,PSP-94,PSP94,Seminal plasma beta-inhibin,MSMB,PRSP,
Full Gene Name: Beta-microseminoprotein
Cellular Localisation: Secreted. Sperm surface. - 基因ID
- 4477
- UniProt
- P08118
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