Thymic Stromal Lymphopoietin ELISA 试剂盒
-
- 抗原 See all Thymic Stromal Lymphopoietin (TSLP) ELISA试剂盒
- Thymic Stromal Lymphopoietin (TSLP)
- 抗原表位
- AA 20-140
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TSLP
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: Y20-E140 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: Y20-E140 - Featured
- Discover our best selling TSLP ELISA Kit
- Top Product
- Discover our top product TSLP ELISA Kit
-
Thymic Stromal Lymphopoietin (TSLP) ELISA Kit
TSLP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitHigh Sensitivity TSLP 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 人 AA 29-159 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 小鼠 Colorimetric Sandwich ELISA 0.02-1.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 人 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse TSLP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TSLP has been precoated onto 96-well plates. Standards(NSO, Y20-E140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSLP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TSLP amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TSLP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TSLP standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 98, Standard deviation: 3.8, CV(%): 3.9
- Sample 2: n=16, Mean(pg/ml): 338, Standard deviation: 13.86, CV(%): 4.1
- Sample 3: n=16, Mean(pg/ml): 764, Standard deviation: 34.38, CV(%): 4.5,
- Sample 1: n=24, Mean(pg/ml): 139, Standard deviation: 6.53, CV(%): 4.7
- Sample 2: n=24, Mean(pg/ml): 462, Standard deviation: 25.87, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 732, Standard deviation: 46.12, CV(%): 6.3
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Thymic Stromal Lymphopoietin (TSLP) ELISA试剂盒
- Thymic Stromal Lymphopoietin (TSLP)
- 别名
- TSLP (TSLP 产品)
- 别名
- TSLP ELISA Kit, thymic stromal lymphopoietin ELISA Kit, TSLP ELISA Kit, Tslp ELISA Kit
- 背景
-
Protein Function: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells (By similarity). .
Background: Thymic stromal lymphopoietin, also called TSLP, is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.
Synonyms: Thymic stromal lymphopoietin,Thymic stroma-derived lymphopoietin,Tslp,
Full Gene Name: Thymic stromal lymphopoietin
- 基因ID
- 53603
- UniProt
- Q9JIE6
-
