Adipsin ELISA 试剂盒
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
- 抗原表位
- AA 26-253
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20.000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CFD
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: I26-A253 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: I26-A253 - Featured
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Complement Factor D (CFD) ELISA Kit
CFD 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor D (CFD) ELISA KitCFD 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Complement Factor D (CFD) ELISA KitCFD 适用: 小鼠 AA 26-259 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Complement Factor D (CFD) ELISA KitCFD 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S1 family.
- 板类型
- Pre-coated
- 实验流程
- human CFD ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFD has been precoated onto 96-well plates. Standards(NSO, I26-A253) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFD is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CFD amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CFD standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CFD standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.05, Standard deviation: 0.071, CV(%): 6.8
- Sample 2: n=16, Mean(ng/ml): 3.42, Standard deviation: 0.195, CV(%): 5.7
- Sample 3: n=16, Mean(ng/ml): 8.67, Standard deviation: 0.52, CV(%): 6,
- Sample 1: n=24, Mean(pg/ml): 1.32, Standard deviation: 0.115, CV(%): 8.7
- Sample 2: n=24, Mean(ng/ml): 3.74, Standard deviation: 0.269, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 8.23, Standard deviation: 0.584, CV(%): 7.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Adipsin (CFD) ELISA试剂盒
- Adipsin (CFD) (Complement Factor D (CFD))
- 别名
- CFD (CFD 产品)
- 别名
- ADIPSIN ELISA Kit, ADN ELISA Kit, DF ELISA Kit, PFD ELISA Kit, Adn ELISA Kit, Df ELISA Kit, EVE ELISA Kit, CFD ELISA Kit, adipsin ELISA Kit, pfd ELISA Kit, cfdl ELISA Kit, wu:fb61f12 ELISA Kit, zgc:109940 ELISA Kit, 10.5 ELISA Kit, 10.9 ELISA Kit, 14.10 ELISA Kit, 20.35 ELISA Kit, CG2328 ELISA Kit, Dmel\\CG2328 ELISA Kit, E(eve) ELISA Kit, Eve ELISA Kit, F ELISA Kit, V ELISA Kit, VI ELISA Kit, eve2 ELISA Kit, even ELISA Kit, l(2)46CFg ELISA Kit, l(2)46CFh ELISA Kit, l(2)46CFj ELISA Kit, l(2)46CFp ELISA Kit, l(2)46Ce ELISA Kit, l(2)46Cg ELISA Kit, complement factor D ELISA Kit, complement factor D (adipsin) ELISA Kit, complement factor D (adipsin) L homeolog ELISA Kit, even skipped ELISA Kit, CFD ELISA Kit, Cfd ELISA Kit, cfd.L ELISA Kit, cfd ELISA Kit, eve ELISA Kit
- 背景
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Protein Function: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Background: Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
Synonyms: Complement factor D,3.4.21.46,Adipsin,C3 convertase activator,Properdin factor D,CFD,DF, PFD,
Full Gene Name: Complement factor D
Cellular Localisation: Secreted. - 基因ID
- 1675
- UniProt
- P00746
- 途径
- Complement System
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