SAA1 ELISA 试剂盒
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- 抗原 See all SAA1 ELISA试剂盒
- SAA1 (Serum Amyloid A1 (SAA1))
- 抗原表位
- AA 20-122
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56-100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAA
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: G20-Y122 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <150pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: G20-Y122 - Featured
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Serum Amyloid A1 (SAA1) ELISA Kit
SAA1 适用: 人 Colorimetric Sandwich ELISA 156 ng/mL - 10000 ng/mL Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 猴 Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 绵羊 Colorimetric Competition ELISA 1.875 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 人 AA 20-122 Colorimetric Sandwich ELISA
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 猴 Colorimetric Sandwich ELISA 0.625-40 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Detected in blood plasma (at protein level). Detected in liver. .
- 板类型
- Pre-coated
- 实验流程
- mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA has been precoated onto 96-well plates. Standards(E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAA amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse SAA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse SAA standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.4, Standard deviation: 0.06, CV(%): 4.3
- Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.177, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.291, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.081, CV(%): 5.4
- Sample 2: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.223, CV(%): 6.2
- Sample 3: n=24, Mean(ng/ml): 6.8, Standard deviation: 0.388, CV(%): 5.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all SAA1 ELISA试剂盒
- SAA1 (Serum Amyloid A1 (SAA1))
- 别名
- SAA1 (SAA1 产品)
- 别名
- PIG4 ELISA Kit, SAA ELISA Kit, SAA2 ELISA Kit, TP53I4 ELISA Kit, zgc:103580 ELISA Kit, SAA1 ELISA Kit, Saa-1 ELISA Kit, Saa2 ELISA Kit, serum amyloid A1 ELISA Kit, serum amyloid A ELISA Kit, amyloid protein A ELISA Kit, serum amyloid A protein ELISA Kit, serum amyloid a protein ELISA Kit, serum amyloid A 1 ELISA Kit, serum amyloid protein A ELISA Kit, SAA1 ELISA Kit, saa ELISA Kit, LOC694827 ELISA Kit, Sden_2438 ELISA Kit, Mmc1_0571 ELISA Kit, Riean_0971 ELISA Kit, Saa1 ELISA Kit, LOC100009168 ELISA Kit, LOC101120204 ELISA Kit
- 背景
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Protein Function: Major acute phase protein. .
Background: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
Synonyms: Serum amyloid A-1 protein,Saa1,
Full Gene Name: Serum amyloid A-1 protein
Cellular Localisation: Secreted. - 基因ID
- 111345
- UniProt
- P05366
- 途径
- Toll-Like Receptors Cascades
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