IL15RA ELISA 试剂盒
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- 抗原 See all IL15RA ELISA试剂盒
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
- 抗原表位
- AA 33-205
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL15RA
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: G33-K205 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: G33-K205 - Featured
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Interleukin 15 Receptor, alpha (IL15RA) ELISA Kit
IL15RA 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 人 Colorimetric Sandwich ELISA 0.08-20 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 15 Receptor, alpha (IL15RA) ELISA KitIL15RA 适用: 小鼠 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Widely expressed. .
- 板类型
- Pre-coated
- 实验流程
- mouse IL15RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for IL15RA has been precoated onto 96-well plates. Standards(NSO, G33-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL15RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL15RA amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 925, Standard deviation: 43.5, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 3080, Standard deviation: 200.2, CV(%): 6.5
- Sample 3: n=16, Mean(pg/ml): 5122, Standard deviation: 276.6, CV(%): 5.4,
- Sample 1: n=24, Mean(pg/ml): 1273, Standard deviation: 65, CV(%): 5.1
- Sample 2: n=24, Mean(pg/ml): 3377, Standard deviation: 236.4, CV(%): 7
- Sample 3: n=24, Mean(pg/ml): 5419, Standard deviation: 341.4, CV(%): 6.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL15RA ELISA试剂盒
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
- 别名
- IL15RA (IL15RA 产品)
- 别名
- IL15RA ELISA Kit, CD215 ELISA Kit, AA690181 ELISA Kit, IL-15RA ELISA Kit, interleukin 15 receptor subunit alpha ELISA Kit, interleukin 15 receptor, alpha chain ELISA Kit, IL15RA ELISA Kit, Il15ra ELISA Kit
- 背景
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Protein Function: High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction involves SYK (By similarity). .
Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.
Synonyms: Interleukin-15 receptor subunit alpha,IL-15 receptor subunit alpha,IL-15R-alpha,IL-15RA,CD215,Soluble interleukin-15 receptor subunit alpha,sIL-15 receptor subunit alpha,sIL-15R-alpha,sIL-15RA,Il15ra,
Full Gene Name: Interleukin-15 receptor subunit alpha
Cellular Localisation: Membrane, Single-pass type I membrane protein . Nucleus membrane, Single-pass type I membrane protein. - 基因ID
- 16169
- UniProt
- Q60819
- 途径
- JAK/STAT Signaling
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