Periostin ELISA 试剂盒
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- 抗原 See all Periostin (POSTN) ELISA试剂盒
- Periostin (POSTN)
- 抗原表位
- AA 22-836
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Periostin/OSF2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: N22-Q836 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: N22-Q836 - Featured
- Discover our best selling POSTN ELISA Kit
- Top Product
- Discover our top product POSTN ELISA Kit
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Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 AA 24-836 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.05-16 ng/mL Cell Culture Supernatant, Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 EMI domain.
Tissue Specificity: Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. .
- 板类型
- Pre-coated
- 实验流程
- human Periostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Periostin has been precoated onto 96-well plates. Standards(NSO, N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Periostin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Periostin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Periostin standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1392, Standard deviation: 90.48, CV(%): 6.5
- Sample 2: n=16, Mean(pg/ml): 2593, Standard deviation: 121.9, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 4831, Standard deviation: 280.2, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 1436, Standard deviation: 107.7, CV(%): 7.5
- Sample 2: n=24, Mean(pg/ml): 2734, Standard deviation: 153, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 5166, Standard deviation: 330.6, CV(%): 6.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis." in: Scientific reports, Vol. 6, pp. 20587, (2016) (PubMed).
: "Dysregulation of astrocyte extracellular signaling in Costello syndrome." in: Science translational medicine, Vol. 7, Issue 286, pp. 286ra66, (2015) (PubMed).
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: "TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis." in: Scientific reports, Vol. 6, pp. 20587, (2016) (PubMed).
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- 抗原 See all Periostin (POSTN) ELISA试剂盒
- Periostin (POSTN)
- 别名
- POSTN (POSTN 产品)
- 别名
- fa99h07 ELISA Kit, pn ELISA Kit, postn ELISA Kit, wu:fa99h07 ELISA Kit, wu:fc70f09 ELISA Kit, zgc:153873 ELISA Kit, LOC100008945 ELISA Kit, osf-2 ELISA Kit, pdlpostn ELISA Kit, periostin ELISA Kit, OSF-2 ELISA Kit, OSF2 ELISA Kit, PDLPOSTN ELISA Kit, PN ELISA Kit, RP11-412K4.1 ELISA Kit, A630052E07Rik ELISA Kit, AI747096 ELISA Kit, Osf2 ELISA Kit, PLF ELISA Kit, peri ELISA Kit, Plf ELISA Kit, periostin ELISA Kit, periostin, osteoblast specific factor b ELISA Kit, periostin, osteoblast specific factor ELISA Kit, POSTN ELISA Kit, postnb ELISA Kit, postn ELISA Kit, Postn ELISA Kit
- 背景
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Protein Function: Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. .
Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Synonyms: Periostin,PN,Osteoblast-specific factor 2,OSF-2,POSTN,OSF2,
Full Gene Name: Periostin
Cellular Localisation: Golgi apparatus . Secreted, extracellular space, extracellular matrix . Colocalizes with BMP1 in the Golgi.. - 基因ID
- 10631
- UniProt
- Q15063
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