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Kallikrein 14 ELISA 试剂盒

KLK14 适用: 人 AA 19-248 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
产品编号 ABIN1889328
发货至: 中国
  • 抗原 See all Kallikrein 14 (KLK14) ELISA试剂盒
    Kallikrein 14 (KLK14)
    抗原表位
    AA 19-248
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein 14
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: Q19-M248
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <2pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: Q19-M248
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

    Tissue Specificity: Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). .

    板类型
    Pre-coated
    实验流程
    human Kallikrein 14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 14 has been precoated onto 96-well plates. Standards(NSO, Q19-M248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 14 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Kallikrein 14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallikrein 14 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 124, Standard deviation: 8.31, CV(%): 6.7
    • Sample 2: n=16, Mean(pg/ml): 364, Standard deviation: 17.5, CV(%): 4.8
    • Sample 3: n=16, Mean(pg/ml): 613, Standard deviation: 32.5, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 10.66, CV(%): 7.3
    • Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 22.44, CV(%): 5.5
    • Sample 3: n=24, Mean(pg/ml): 644, Standard deviation: 39.3, CV(%): 6.1
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all Kallikrein 14 (KLK14) ELISA试剂盒
    Kallikrein 14 (KLK14)
    别名
    KLK14 (KLK14 产品)
    别名
    GK14 ELISA Kit, KLK-L6 ELISA Kit, kallikrein related-peptidase 14 ELISA Kit, kallikrein related peptidase 14 ELISA Kit, Klk14 ELISA Kit, KLK14 ELISA Kit
    背景

    Protein Function: Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. .

    Background: Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.

    Synonyms: Kallikrein-14,hK14,3.4.21.-,Kallikrein-like protein 6,KLK-L6,KLK14,KLKL6,

    Full Gene Name: Kallikrein-14

    Cellular Localisation: Secreted, extracellular space.
    基因ID
    9622
    UniProt
    Q9P0G3
    途径
    Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling
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