MME ELISA 试剂盒
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
- 抗原表位
- AA 52-750
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD10/Neprilysin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: Y52-W750 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: Y52-W750 - Featured
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- Discover our top product MME ELISA Kit
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Membrane Metallo-Endopeptidase (MME) ELISA Kit
MME 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 AA 52-750 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 61-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
SensoLyte® 520 Neprilysin Activity Assay KitAct, FRET Fluorometric
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase M13 family.
- 板类型
- Pre-coated
- 实验流程
- human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD10 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1265, Standard deviation: 48.07, CV(%): 3.8
- Sample 2: n=16, Mean(pg/ml): 3210, Standard deviation: 179.8, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 6840, Standard deviation: 321.5, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 1526, Standard deviation: 83.93, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 2761, Standard deviation: 201, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 6397, Standard deviation: 369.6, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
- 别名
- MME (MME 产品)
- 别名
- CALLA ELISA Kit, CD10 ELISA Kit, NEP ELISA Kit, SFE ELISA Kit, 6030454K05Rik ELISA Kit, C85356 ELISA Kit, Nep ELISA Kit, neprilysin ELISA Kit, membrane metalloendopeptidase ELISA Kit, membrane metallo endopeptidase ELISA Kit, membrane metallo-endopeptidase ELISA Kit, MME ELISA Kit, Mme ELISA Kit
- 物质类
- Chemical
- 背景
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Protein Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). .
Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
Synonyms: Neprilysin,3.4.24.11 ,Atriopeptidase,Common acute lymphocytic leukemia antigen,CALLA,Enkephalinase,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Skin fibroblast elastase,SFE,CD10,MME,EPN,
Full Gene Name: Neprilysin
Cellular Localisation: Cell membrane, Single-pass type II membrane protein. - 基因ID
- 4311
- UniProt
- P08473
- 途径
- RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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