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IGFBP2 ELISA 试剂盒

IGFBP2 适用: 人 AA 40-328 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
产品编号 ABIN1889294
发货至: 中国
  • 抗原 See all IGFBP2 ELISA试剂盒
    IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
    抗原表位
    AA 40-328
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5-4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP2
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: E40-Q328
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: E40-Q328
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    板类型
    Pre-coated
    实验流程
    human IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E40-Q328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP2 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin or EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP2 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 608, Standard deviation: 27.97, CV(%): 4.6
    • Sample 2: n=16, Mean(pg/ml): 1555, Standard deviation: 80.86, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 2836, Standard deviation: 167.3, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 715, Standard deviation: 44.33, CV(%): 6.2
    • Sample 2: n=24, Mean(pg/ml): 1938, Standard deviation: 133.7, CV(%): 6.9
    • Sample 3: n=24, Mean(pg/ml): 3147, Standard deviation: 229.7, CV(%): 7.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all IGFBP2 ELISA试剂盒
    IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
    别名
    IGFBP2 (IGFBP2 产品)
    别名
    IBP2 ELISA Kit, IGF-BP53 ELISA Kit, AI255832 ELISA Kit, IBP-2 ELISA Kit, Igfbp-2 ELISA Kit, mIGFBP-2 ELISA Kit, IGFBP-2 ELISA Kit, ILGFBPA ELISA Kit, pIGFBP-2 ELISA Kit, igfbp2 ELISA Kit, MGC65741 ELISA Kit, MGC76823 ELISA Kit, MGC174445 ELISA Kit, IGFBP2 ELISA Kit, ibp2 ELISA Kit, igf-bp53 ELISA Kit, igfbp2a ELISA Kit, si:ch211-2k18.3 ELISA Kit, insulin like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2a ELISA Kit, insulin-like growth factor binding protein 2b ELISA Kit, IGFBP2 ELISA Kit, Igfbp2 ELISA Kit, igfbp2a ELISA Kit, igfbp2 ELISA Kit, igfbp2b ELISA Kit
    背景

    Protein Function: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

    Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

    Synonyms: Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2,IGFBP2,BP2, IBP2,

    Full Gene Name: Insulin-like growth factor-binding protein 2

    Cellular Localisation: Secreted.
    基因ID
    3485
    UniProt
    P18065
    途径
    Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation
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