FLT3LG ELISA 试剂盒
-
- 抗原 See all FLT3LG ELISA试剂盒
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 抗原表位
- AA 27-185
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Flt-3ligand
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: sf21
Immunogen sequence: T27-P185 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: sf21
Immunogen sequence: T27-P185 - Featured
- Discover our best selling FLT3LG ELISA Kit
- Top Product
- Discover our top product FLT3LG ELISA Kit
-
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA Kit
FLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 AA 27-188 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人, 猕猴 Colorimetric Sandwich ELISA 12-400 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3ligand has been precoated onto 96-well plates. Standards(sf21, T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Flt-3ligand amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 109, Standard deviation: 3.49, CV(%): 3.2
- Sample 2: n=16, Mean(pg/ml): 256, Standard deviation: 8.45, CV(%): 3.3
- Sample 3: n=16, Mean(pg/ml): 520, Standard deviation: 22.88, CV(%): 4.4,
- Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 10.64, CV(%): 9.5
- Sample 2: n=24, Mean(pg/ml): 245, Standard deviation: 18.38, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 41.96, CV(%): 8.1
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all FLT3LG ELISA试剂盒
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- 别名
- FLT3LG (FLT3LG 产品)
- 别名
- Flt3lg ELISA Kit, Ly72L ELISA Kit, FL ELISA Kit, FLT3L ELISA Kit, Vegfr3 ELISA Kit, fms related tyrosine kinase 3 ligand ELISA Kit, FMS-like tyrosine kinase 3 ligand ELISA Kit, fms-related tyrosine kinase 4 ELISA Kit, fms-related tyrosine kinase 3 ligand ELISA Kit, FLT3LG ELISA Kit, Flt3l ELISA Kit, Flt4 ELISA Kit, Flt3lg ELISA Kit
- 背景
-
Protein Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Synonyms: Fms-related tyrosine kinase 3 ligand,Flt3 ligand,Flt3L,SL cytokine,FLT3LG,
Full Gene Name: Fms-related tyrosine kinase 3 ligand
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 2323
- UniProt
- P49771
- 途径
- RTK signaling
-
