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- 抗原 See all SIRT2 试剂盒
- SIRT2 (Sirtuin 2 (SIRT2))
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® 520 FRET SIRT2 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 2 activity using FRET substrate. In the first step, an acetylated substrate is incubated with SIRT2 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490/520 nm. This kit can be used for high throughput screening of SIRT2 inhibitors/activators. The long wavelength fluorescence of FRET substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well plate format. 100 assays (96-well plate)
- Top Product
- Discover our top product SIRT2 ELISA Kit
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Sirtuin 2 (SIRT2) ELISA Kit
SIRT2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 2 (SIRT2) ELISA KitSIRT2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 2 (SIRT2) ELISA KitSIRT2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
SensoLyte® Green SIRT2 Assay KitFRET Fluorometric
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.
- 储存条件
- -80 °C
- 储存方法
- Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.
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- 抗原
- SIRT2 (Sirtuin 2 (SIRT2))
- 别名
- SIRT2 (SIRT2 产品)
- 别名
- CG5085 Kit, D.mel2 Kit, Dmel\\CG5085 Kit, SIRT2 Kit, dSIRT2 Kit, sir2 Kit, Sirt2 Kit, BmSirt2 Kit, AtSRT2 Kit, SIRTUIN 2 Kit, T2K12.8 Kit, sirtuin 2 Kit, SIR2 Kit, SIR2L Kit, SIR2L2 Kit, 5730427M03Rik Kit, Sir2l Kit, wu:fb57d05 Kit, zgc:55966 Kit, zgc:77003 Kit, Sirtuin 2 Kit, sirtuin 2 Kit, sirtuin 2 S homeolog Kit, NAD-dependent deacetylase sirtuin 2 homolog Kit, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) Kit, Sirt2 Kit, SIRT2 Kit, sirt2.S Kit, SRT2 Kit, sirt2 Kit
- 背景
- Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD+ as a cofactor. Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.
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