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SensoLyte® 520 MMP-8 Assay Kit

FRET Fluorometric
产品编号 ABIN1882526
发货至: 中国
  • 抗原 See all MMP8 试剂盒
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    检测方法
    Fluorometric
    应用范围
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    品牌
    SensoLyte®
    产品特性
    SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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    Discover our top product MMP8 ELISA Kit
  • 说明

    FRET-based Assay Kit

    限制
    仅限研究用
  • 注意事项
    Protect Components A and B from light.
    储存条件
    -20 °C
    储存方法
    Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
  • Schubert-Unkmeir, Konrad, Slanina, Czapek, Hebling, Frosch: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).

    Slanina, König, Claus, Frosch, Schubert-Unkmeir: "Real-time impedance analysis of host cell response to meningococcal infection." in: Journal of microbiological methods, Vol. 84, Issue 1, pp. 101-8, (2010) (PubMed).

  • 抗原
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    别名
    MMP-8 (MMP8 产品)
    别名
    MMP8 Kit, BB138268 Kit, CLG1 Kit, HNC Kit, MMP-8 Kit, PMNL-CL Kit, matrix metallopeptidase 8 Kit, neutrophil collagenase Kit, MMP8 Kit, Mmp8 Kit, LOC100339790 Kit
    背景
    Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.
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