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Quick Overview for SensoLyte® 520 MMP-3 Assay Kit (ABIN1882522)
抗原
See all MMP3 试剂盒检测方法
应用范围
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产品特性
- SensoLyte® 520 MMP-3 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-3 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-3, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA Kit
MMP3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 人 AA 18-477 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 AA 18-477 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Pig Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "IL-1?-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway." in: Experimental cell research, Vol. 328, Issue 1, pp. 69-86, (2014) (PubMed).
: "Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament ..." in: The American journal of sports medicine, Vol. 42, Issue 5, pp. 1211-8, (2014) (PubMed).
: "Proinflammatory cytokines induce stromelysin-1-mediated cell proliferation in dental pulp fibroblast-like cells." in: Journal of endodontics, Vol. 40, Issue 1, pp. 89-94, (2013) (PubMed).
: "Matrix metalloproteinase-3 is activated by HtrA2/Omi in dopaminergic cells: relevance to Parkinson's disease." in: Neurochemistry international, Vol. 60, Issue 3, pp. 249-56, (2012) (PubMed).
: "Liver regeneration associated protein (ALR) exhibits antimetastatic potential in hepatocellular carcinoma." in: Molecular medicine (Cambridge, Mass.), Vol. 17, Issue 3-4, pp. 221-8, (2011) (PubMed).
: "Endothelins stimulate the production of stromelysin-1 in cultured rat astrocytes." in: Biochemical and biophysical research communications, Vol. 371, Issue 4, pp. 659-63, (2008) (PubMed).
: "Inhibition of MMP-3 or -9 suppresses lipopolysaccharide-induced expression of proinflammatory cytokines and iNOS in microglia." in: Journal of neurochemistry, Vol. 106, Issue 2, pp. 770-80, (2008) (PubMed).
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- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
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别名
- MMP-3
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion and other diseases is well known. MMP-3 (stromelysin-1, transin-1) is proposed as a potential therapeutic target.
抗原
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