(6 references)
产品编号 ABIN1882502
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for SensoLyte® Generic MMP Assay Kit (ABIN1882502)
抗原
Matrix Metalloproteinase
检测方法
Colorimetric
应用范围
Enzyme Activity Assay (EAA)
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产品特性
- The SensoLyte® Generic MMP Assay Kit can be used to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 or for high throughput screening of MMPs' inducers and inhibitors. The substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group, which can be detected with a color-developing thiol-reactive agent, 4,4'-dithiodipyridine or Ellman's Reagent at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.
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SensoLyte® 520 Generic MMP Activity Kit
FRET, Act Fluorometric
SensoLyte® 570 Generic MMP Assay KitFRET Fluorometric
SensoLyte® 520 MMP Substrate Sampler KitFRET Fluorometric
SensoLyte® 520 MMP Profiling KitFRET Fluorometric
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说明
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Enzymatic Activity Assay kit
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限制
- 仅限研究用
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注意事项
- Aliquot Components E and F as needed to avoid freeze-thaw cycles. Protect from light.
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储存条件
- -20 °C
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储存方法
- Store all kit components at -20 °C. Store Component C at 4 °C in the dark. Component G can be stored at room temperature for convenience.
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: "A dynamic model of calcific nodule destabilization in response to monocyte- and oxidized lipid-induced matrix metalloproteinases." in: American journal of physiology. Cell physiology, Vol. 302, Issue 4, pp. C658-65, (2012) (PubMed).
: "Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 22, Issue 3, pp. 725-34, (2012) (PubMed).
: "Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro." in: Journal of dental research, Vol. 91, Issue 2, pp. 192-6, (2012) (PubMed).
: "The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs." in: Journal of dental research, Vol. 90, Issue 4, pp. 535-40, (2011) (PubMed).
: "Inhibition of endogenous dentin matrix metalloproteinases by ethylenediaminetetraacetic acid." in: Journal of endodontics, Vol. 38, Issue 1, pp. 62-5, (2011) (PubMed).
: "The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin." in: Acta biomaterialia, Vol. 6, Issue 10, pp. 4136-42, (2010) (PubMed).
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: "A dynamic model of calcific nodule destabilization in response to monocyte- and oxidized lipid-induced matrix metalloproteinases." in: American journal of physiology. Cell physiology, Vol. 302, Issue 4, pp. C658-65, (2012) (PubMed).
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- Matrix Metalloproteinase
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别名
- MMP
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.
抗原
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