HABA Biotin Quantitation Kit
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- 抗原 See all Biotin products
- Biotin
- 检测方法
- Colorimetric
- 标记
- Biotin
- 应用范围
- Enzyme Activity Assay (EAA)
- 产品特性
- The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.
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- 说明
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Enzymatic Activity Assay kit
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store Component A at -20 °C. Store all other components at 4 °C
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
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