SensoLyte® 520 Factor Xa Assay Kit
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- 抗原 See all Factor Xa (FXa) 试剂盒
- Factor Xa (FXa) (Activated Coagulation Factor X (FXa))
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® 520 Factor Xa Assay Kit provides a convenient assay for high throughput screening of FXa inhibitors or continuous assay of enzyme activity. It detects FXa activity using a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage into two separate fragments by FXa, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. The assays are performed in a convenient 96-well microplate format.
- Top Product
- Discover our top product FXa ELISA Kit
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Protect Components A and B from light and moisture.
- 储存条件
- -80 °C
- 储存方法
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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- 抗原
- Factor Xa (FXa) (Activated Coagulation Factor X (FXa))
- 别名
- Factor Xa (FXa 产品)
- 别名
- FX Kit, FXA Kit, coagulation factor X Kit, F10 Kit
- 背景
- Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.
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