(32 references)-
- 抗原 See all Caspase 3 (CASP3) 试剂盒
- Caspase 3 (CASP3)
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte®Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
- Top Product
- Discover our top product CASP3 ELISA Kit
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Caspase 3 (CASP3) ELISA Kit
CASP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 3 (CASP3) ELISA KitCASP3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 3 (CASP3) ELISA KitCASP3 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Keep Components A and B away from light
- 储存条件
- -20 °C
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: "EGF-like ligands mediate progesterone's anti-apoptotic action on macaque granulosa cells." in: Biology of reproduction, Vol. 88, Issue 1, pp. 18, (2013) (PubMed).
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: "A relatively low level of ribosome depurination by mutant forms of ricin toxin A chain can trigger protein synthesis inhibition, cell signaling and apoptosis in mammalian cells." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 12, pp. 2204-11, (2012) (PubMed).
: "The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 16, pp. 6283-8, (2012) (PubMed).
: "Intravenous ferumoxytol allows noninvasive MR imaging monitoring of macrophage migration into stem cell transplants." in: Radiology, Vol. 264, Issue 3, pp. 803-11, (2012) (PubMed).
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: "The histone deacetylase inhibitor valproic acid sensitizes human and canine osteosarcoma to doxorubicin." in: Cancer chemotherapy and pharmacology, Vol. 67, Issue 1, pp. 83-92, (2011) (PubMed).
: "Fe(III)-salen and salphen complexes induce caspase activation and apoptosis in human cells." in: Journal of biomolecular screening, Vol. 16, Issue 1, pp. 26-35, (2011) (PubMed).
: "The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines." in: BMC cancer, Vol. 11, pp. 112, (2011) (PubMed).
: "Inhibition of proprotein convertase SKI-1 blocks transcription of key extracellular matrix genes regulating osteoblastic mineralization." in: The Journal of biological chemistry, Vol. 286, Issue 3, pp. 1836-49, (2011) (PubMed).
: "Interleukin-27 and interferon-gamma are involved in regulation of autoimmune arthritis." in: The Journal of biological chemistry, Vol. 286, Issue 4, pp. 2817-25, (2011) (PubMed).
: "Herbal formula CGX ameliorates LPS/D-galactosamine-induced hepatitis." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 49, Issue 6, pp. 1329-34, (2011) (PubMed).
: "Identification of components of grape powder with anti-apoptotic effects." in: Toxicology and industrial health, Vol. 27, Issue 1, pp. 19-28, (2011) (PubMed).
: "Hydrogen peroxide inhibits mTOR signaling by activation of AMPKalpha leading to apoptosis of neuronal cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 5, pp. 762-73, (2010) (PubMed).
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: "Acquired tumor cell radiation resistance at the treatment site is mediated through radiation-orchestrated intercellular communication." in: International journal of radiation oncology, biology, physics, Vol. 88, Issue 3, pp. 677-85, (2014) (PubMed).
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- 抗原
- Caspase 3 (CASP3)
- 别名
- Caspase-3 (CASP3 产品)
- 别名
- CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
- 背景
- Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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