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SensoLyte® Biotin Quantitation Kit

FRET Fluorometric
产品编号 ABIN1882421
发货至: 中国
  • 抗原 See all Biotin products
    Biotin
    检测方法
    Fluorometric
    标记
    Biotin
    应用范围
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    品牌
    SensoLyte®
    产品特性
    The SensoLyte® Biotin Quantitation Assay Kit provides a convenient assay for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantifying biotin concentration in a solution. The assay is based on FRET principle. In the first step, avidin, Reagent A tagged with a quencher dye is briefly incubated with biotin samples/standards. In the second step, fluorescent biotin, Reagent B, is added to the assay mixture. The fluorescent signal is directly proportional to the amount of biotin in the sample and can be detected at Ex/Em=490/520 nm. The kit is best used to determine biotin concentration in the range from 2 to 20 picomoles. The kit also provides biotinylated IgG for use as a positive control and a protease for protein digestion to expose hidden biotin groups.
  • 说明

    FRET-based Assay Kit

    限制
    仅限研究用
  • 注意事项
    Protect Components A and B from light and from moisture.
    储存条件
    -20 °C
    储存方法
    Store all kit components at -20 °C. Components E can be stored at room temperature for convenience.
  • 抗原
    Biotin
    Abstract
    Biotin 产品
    物质类
    Chemical
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