IL17F ELISA 试剂盒
(1 reference)-
- 抗原 See all IL17F ELISA试剂盒
- IL17F (Interleukin 17F (IL17F))
- 抗原表位
- AA 19-153
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-17F
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A19-A153 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A19-A153 - Featured
- Discover our best selling IL17F ELISA Kit
- Top Product
- Discover our top product IL17F ELISA Kit
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Interleukin 17F (IL17F) ELISA Kit
IL17F 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 AA 21-153 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the IL-17 family.
- 板类型
- Pre-coated
- 实验流程
- rat IL-17F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17F has been precoated onto 96-well plates. Standards(E.coli, A19-A153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-17F amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat IL-17F standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-17F standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 275, Standard deviation: 12.93, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 524, Standard deviation: 32.5, CV(%): 6.2
- Sample 3: n=16, Mean(pg/ml): 928, Standard deviation: 52, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 352, Standard deviation: 17.6, CV(%): 5
- Sample 2: n=24, Mean(pg/ml): 723, Standard deviation: 52.78, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 1230, Standard deviation: 83.64, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 404-12, (2005) (PubMed).
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: "Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 404-12, (2005) (PubMed).
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- 抗原 See all IL17F ELISA试剂盒
- IL17F (Interleukin 17F (IL17F))
- 别名
- IL17F (IL17F 产品)
- 别名
- CANDF6 ELISA Kit, IL-17F ELISA Kit, ML-1 ELISA Kit, ML1 ELISA Kit, C87042 ELISA Kit, IL17F ELISA Kit, interleukin 17F ELISA Kit, IL17F ELISA Kit, Il17f ELISA Kit
- 背景
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Protein Function: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis (By similarity). .
Background: Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
Synonyms: Interleukin-17F,IL-17F,Il17f,
Full Gene Name: Interleukin-17F
Cellular Localisation: Secreted. - 基因ID
- 301291
- UniProt
- Q5BJ95
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
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