IL1RL1 ELISA 试剂盒
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- 抗原 See all IL1RL1 ELISA试剂盒
- IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
- 抗原表位
- AA 19-328
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1RL1/ST2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: K19-S328 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: K19-S328 - Featured
- Discover our best selling IL1RL1 ELISA Kit
- Top Product
- Discover our top product IL1RL1 ELISA Kit
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Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA Kit
IL1RL1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitSmall Sample IL1RL1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 小鼠 AA 27-332 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor-Like 1 (IL1RL1) ELISA KitIL1RL1 适用: 人 Colorimetric Sandwich ELISA Plasma (EDTA), Serum, Tissue Culture Medium
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the interleukin-1 receptor family.
Tissue Specificity: Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. .
- 板类型
- Pre-coated
- 实验流程
- human IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1RL1 has been precoated onto 96-well plates. Standards(NSO, K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1RL1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL1RL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL1RL1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 0.53, Standard deviation: 0.028, CV(%): 5.2
- Sample 2: n=16, Mean(ng/ml): 2.05, Standard deviation: 0.094, CV(%): 4.6
- Sample 3: n=16, Mean(ng/ml): 4.31, Standard deviation: 0.142, CV(%): 3.3,
- Sample 1: n=24, Mean(ng/ml): 0.69, Standard deviation: 0.06, CV(%): 8.7
- Sample 2: n=24, Mean(ng/ml): 2.54, Standard deviation: 0.157, CV(%): 6.2
- Sample 3: n=24, Mean(ng/ml): 4.68, Standard deviation: 0.229, CV(%): 4.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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-
: "IL-33 and ST2 levels in chronic kidney disease: Associations with inflammation, vascular abnormalities, cardiovascular events, and survival." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178939, (2017) (PubMed).
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: "IL-33 and ST2 levels in chronic kidney disease: Associations with inflammation, vascular abnormalities, cardiovascular events, and survival." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178939, (2017) (PubMed).
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- 抗原 See all IL1RL1 ELISA试剂盒
- IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
- 别名
- IL1RL1 (IL1RL1 产品)
- 别名
- DER4 ELISA Kit, Fit-1 ELISA Kit, Ly84 ELISA Kit, ST2L ELISA Kit, St2 ELISA Kit, St2-rs1 ELISA Kit, T1 ELISA Kit, T1/ST2 ELISA Kit, FIT-1 ELISA Kit, IL33R ELISA Kit, ST2 ELISA Kit, ST2V ELISA Kit, FIT1 ELISA Kit, interleukin 1 receptor-like 1 ELISA Kit, interleukin 1 receptor like 1 ELISA Kit, Il1rl1 ELISA Kit, IL1RL1 ELISA Kit
- 背景
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Protein Function: Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. .
Background: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
Synonyms: Interleukin-1 receptor-like 1,Protein ST2,IL1RL1,DER4, ST2, T1,
Full Gene Name: Interleukin-1 receptor-like 1
Cellular Localisation: Isoform C: Cell membrane. - 基因ID
- 9173
- UniProt
- Q01638
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