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FASL ELISA 试剂盒

FASL 适用: 大鼠 AA 127-278 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
产品编号 ABIN1672899
发货至: 中国
  • 抗原 See all FASL ELISA试剂盒
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    抗原表位
    AA 127-278
    适用
    • 13
    • 9
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat FASL
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: Q127-L278
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: Q127-L278
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Expressed in activated splenocytes and thymocytes. Moderate or weak expression found in small intestines, kidney and lung.

    板类型
    Pre-coated
    实验流程
    rat FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FAS-L amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FAS-L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat FAS-L standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 118, Standard deviation: 4.13, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 854, Standard deviation: 40.99, CV(%): 4.8
    • Sample 3: n=16, Mean(pg/ml): 1319, Standard deviation: 71.23, CV(%): 5.4,
    • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 5.2, CV(%): 5.1
    • Sample 2: n=24, Mean(pg/ml): 847, Standard deviation: 52.51, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 1423, Standard deviation: 103.9, CV(%): 7.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

    Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

  • 抗原 See all FASL ELISA试剂盒
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    别名
    FASLG (FASL 产品)
    别名
    ALPS1B ELISA Kit, APT1LG1 ELISA Kit, APTL ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, fasl ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, Apt1Lg1 ELISA Kit, Fasl ELISA Kit, FASLG ELISA Kit, zgc:162027 ELISA Kit, Fas ligand ELISA Kit, Fas ligand L homeolog ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, FASLG ELISA Kit, faslg.L ELISA Kit, Fasl ELISA Kit, Faslg ELISA Kit, faslg ELISA Kit
    背景

    Protein Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects (By similarity). .

    Background: FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

    Synonyms: Tumor necrosis factor ligand superfamily member 6,CD95 ligand,CD95-L,Fas antigen ligand,Fas ligand,FasL,CD178,Tumor necrosis factor ligand superfamily member 6, membrane form,Tumor necrosis factor ligand superfamily member 6, soluble form,Receptor-binding FasL ectodomain,Soluble Fas ligand,sFasL,ADAM10-processed FasL form,APL,FasL intracellular domain,FasL ICD,SPPL2A-processed FasL form,SPA,Faslg,Apt1Lg1, Cd95l, Fasl, Tnfsf6,

    Full Gene Name: Tumor necrosis factor ligand superfamily member 6

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Colocalizes with the SPPL2A protease at the cell membrane. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (By similarity)..
    基因ID
    25385
    UniProt
    P36940
    途径
    Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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