CD166 ELISA 试剂盒
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- 抗原 See all CD166 (ALCAM) ELISA试剂盒
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
- 抗原表位
- AA 28-526
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ALCAM
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: W28-A526 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: W28-A526 - Featured
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- Discover our top product ALCAM ELISA Kit
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Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit
ALCAM 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 兔 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 AA 28-527 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 AA 28-527 Colorimetric Sandwich ELISA
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 Colorimetric Sandwich ELISA 0.468 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Tissue Specificity: Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. .
- 板类型
- Pre-coated
- 实验流程
- human ALCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ALCAM has been precoated onto 96-well plates. Standards(NSO, W28-A526) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ALCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ALCAM amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human ALCAM standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 3.07, Standard deviation: 0.150, CV(%): 4.9
- Sample 2: n=16, Mean(ng/ml): 6.42, Standard deviation: 0.327, CV(%): 5.1
- Sample 3: n=16, Mean(ng/ml): 13.26, Standard deviation: 0.756, CV(%): 5.7,
- Sample 1: n=24, Mean(ng/ml): 3.58, Standard deviation: 0.218, CV(%): 6.1
- Sample 2: n=24, Mean(ng/ml): 7.04, Standard deviation: 0.444, CV(%): 6.3
- Sample 3: n=24, Mean(ng/ml): 14.98, Standard deviation: 0.959, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CD166 (ALCAM) ELISA试剂盒
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
- 别名
- ALCAM (ALCAM 产品)
- 别名
- CD166 ELISA Kit, MEMD ELISA Kit, ALCAM ELISA Kit, BEN ELISA Kit, DM-GRASP ELISA Kit, JC7 ELISA Kit, SC1 ELISA Kit, AI853494 ELISA Kit, MuSC ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, memd ELISA Kit, fb53h09 ELISA Kit, fb80a10 ELISA Kit, nlcam ELISA Kit, si:ch211-190h2.4 ELISA Kit, wu:fb53h09 ELISA Kit, wu:fb80a10 ELISA Kit, wu:fc14b01 ELISA Kit, activated leukocyte cell adhesion molecule ELISA Kit, activated leukocyte cell adhesion molecule a ELISA Kit, activated leukocyte cell adhesion molecule L homeolog ELISA Kit, CD166 antigen homolog ELISA Kit, activated leukocyte cell adhesion molecule b ELISA Kit, ALCAM ELISA Kit, Alcam ELISA Kit, alcama ELISA Kit, alcam.L ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, alcamb ELISA Kit
- 背景
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Protein Function: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
Synonyms: CD166 antigen,Activated leukocyte cell adhesion molecule,CD166,ALCAM,MEMD,
Full Gene Name: CD166 antigen
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 214
- UniProt
- Q13740
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