LYVE1 ELISA 试剂盒
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- 抗原 See all LYVE1 ELISA试剂盒
- LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
- 抗原表位
- AA 24-238
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human LYVE-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: S24-T238 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: S24-T238 - Featured
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Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) ELISA Kit
LYVE1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) ELISA KitLYVE1 适用: 人 Colorimetric Sandwich ELISA 19.5 pg/mL - 1250 pg/mL Plasma, Serum, Tissue Homogenate
Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) ELISA KitLYVE1 适用: 人 Colorimetric Sandwich ELISA 8-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) ELISA KitLYVE1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) ELISA KitLYVE1 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Mainly expressed in endothelial cells lining lymphatic vessels. .
- 板类型
- Pre-coated
- 实验流程
- human LYVE-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LYVE-1 has been precoated onto 96-well plates. Standards(NSO, S24-T238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LYVE-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LYVE-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human LYVE-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human LYVE-1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 194, Standard deviation: 6.984, CV(%): 3.6
- Sample 2: n=16, Mean(pg/ml): 633, Standard deviation: 29.8, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 1226, Standard deviation: 79.69, CV(%): 6.5,
- Sample 1: n=24, Mean(pg/ml): 255, Standard deviation: 13.26, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 812, Standard deviation: 47.91, CV(%): 5.9
- Sample 3: n=24, Mean(pg/ml): 1438, Standard deviation: 102.1, CV(%): 7.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 14, (2014) (PubMed).
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: "VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 14, (2014) (PubMed).
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- 抗原 See all LYVE1 ELISA试剂盒
- LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
- 别名
- LYVE1 (LYVE1 产品)
- 背景
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Protein Function: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. .
Background: Extracellular link domain containing 1, also known as LYVE-1 or XLKD1 is a human gene. The International Radiation Hybrid Mapping Consortium mapped the LYVE1 gene to chromosome 11p15.4. This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. This gene may play a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding(CRS). It may act as a hyaluronan(HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Synonyms: Lymphatic vessel endothelial hyaluronic acid receptor 1,LYVE-1,Cell surface retention sequence-binding protein 1,CRSBP-1,Extracellular link domain-containing protein 1,Hyaluronic acid receptor,LYVE1,CRSBP1, HAR, XLKD1,UNQ230/PRO263,
Full Gene Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
Cellular Localisation: Membrane, Single-pass type I membrane protein . Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand- dependent internalization and recycling at the cell surface. - 基因ID
- 10894
- UniProt
- Q9Y5Y7
- 途径
- Glycosaminoglycan Metabolic Process
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