IGFBP6 ELISA 试剂盒
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- 抗原 See all IGFBP6 ELISA试剂盒
- IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
- 抗原表位
- AA 25-240
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP6
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A25-G240 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A25-G240 - Featured
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Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kit
IGFBP6 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA KitIGFBP6 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA KitIGFBP6 适用: 马 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA KitIGFBP6 适用: 犬 Colorimetric Competition ELISA 1.0-25 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA KitIGFBP6 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA KitIGFBP6 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 IGFBP N-terminal domain.
- 板类型
- Pre-coated
- 实验流程
- human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NSO, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP6 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 551, Standard deviation: 21.5, CV(%): 3.9
- Sample 2: n=16, Mean(pg/ml): 1557, Standard deviation: 63.84, CV(%): 4.1
- Sample 3: n=16, Mean(pg/ml): 2736, Standard deviation: 128.6, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 489, Standard deviation: 27.87, CV(%): 5.7
- Sample 2: n=24, Mean(pg/ml): 1635, Standard deviation: 101.37, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 2878, Standard deviation: 184.2, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IGFBP6 ELISA试剂盒
- IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
- 别名
- IGFBP6 (IGFBP6 产品)
- 别名
- IBP6 ELISA Kit, IGFBP-6 ELISA Kit, IGF-BP6 ELISA Kit, RBP6A ELISA Kit, IGFBP6 ELISA Kit, igfbp6 ELISA Kit, insulin like growth factor binding protein 6 ELISA Kit, insulin-like growth factor binding protein 6 ELISA Kit, IGFBP6 ELISA Kit, Igfbp6 ELISA Kit, IGFBP-6 ELISA Kit, igfbp6 ELISA Kit
- 背景
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Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Background: Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
Synonyms: Insulin-like growth factor-binding protein 6,IBP-6,IGF-binding protein 6,IGFBP-6,IGFBP6,IBP6,
Full Gene Name: Insulin-like growth factor-binding protein 6
Cellular Localisation: Secreted. - 基因ID
- 3489
- UniProt
- P24592
- 途径
- WNT signaling, Myometrial Relaxation and Contraction
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