Myeloperoxidase ELISA 试剂盒
(7 references)-
- 抗原 See all Myeloperoxidase (MPO) ELISA试剂盒
- Myeloperoxidase (MPO)
- 抗原表位
- AA 16-718
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MPO/Myeloperoxidase
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: M16-T718 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: M16-T718 - Featured
- Discover our best selling MPO ELISA Kit
- Top Product
- Discover our top product MPO ELISA Kit
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Myeloperoxidase (MPO) ELISA Kit
MPO 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 马 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Myeloperoxidase (MPO) ELISA KitMPO 适用: 犬 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 人 AA 49-745 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Homogenate, Urine
Myeloperoxidase (MPO) ELISA KitMPO 适用: 兔 Colorimetric Competition ELISA 1.25-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse MPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MPO has been precoated onto 96-well plates. Standards(NSO, M16-T718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MPO amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MPO standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1248, Standard deviation: 56.16, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 3528, Standard deviation: 179.93, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 6243, Standard deviation: 387.1, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 1425, Standard deviation: 84.1, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 4127, Standard deviation: 247.6, CV(%): 6
- Sample 3: n=24, Mean(pg/ml): 6785, Standard deviation: 468.2, CV(%): 6.9
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice." in: Oncotarget, Vol. 7, Issue 38, pp. 61643-61655, (2018) (PubMed).
: "MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor." in: Oncotarget, Vol. 8, Issue 1, pp. 705-721, (2018) (PubMed).
: "A novel pH-controlled hydrogen sulfide donor protects gastric mucosa from aspirin-induced injury." in: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2441-2451, (2018) (PubMed).
: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).
: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).
: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).
: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).
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: "Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice." in: Oncotarget, Vol. 7, Issue 38, pp. 61643-61655, (2018) (PubMed).
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- 抗原 See all Myeloperoxidase (MPO) ELISA试剂盒
- Myeloperoxidase (MPO)
- 别名
- MPO (MPO 产品)
- 别名
- mKIAA4033 ELISA Kit, MPO ELISA Kit, LOC100335032 ELISA Kit, POX2' ELISA Kit, XPOX2' ELISA Kit, mpo ELISA Kit, mpo-A ELISA Kit, pmr-1 ELISA Kit, pmr1 ELISA Kit, pox2 ELISA Kit, xpox2 ELISA Kit, myeloperoxidase ELISA Kit, myeloperoxidase L homeolog ELISA Kit, MPO ELISA Kit, Mpo ELISA Kit, LOC100335032 ELISA Kit, mpo.L ELISA Kit
- 背景
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Protein Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (By similarity). .
Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase light chain,Myeloperoxidase heavy chain,Mpo,
Full Gene Name: Myeloperoxidase
Cellular Localisation: Lysosome. - 基因ID
- 17523
- UniProt
- P11247
- 途径
- Chromatin Binding
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