Angiopoietin 2 ELISA 试剂盒
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- 抗原 See all Angiopoietin 2 (ANGPT2) ELISA试剂盒
- Angiopoietin 2 (ANGPT2)
- 抗原表位
- AA 1-496
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Angiopoietin-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: M1-F496 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: M1-F496 - Featured
- Discover our best selling ANGPT2 ELISA Kit
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- Discover our top product ANGPT2 ELISA Kit
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Angiopoietin 2 (ANGPT2) ELISA Kit
ANGPT2 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 人 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: Pig Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Angiopoietin 2 (ANGPT2) ELISA KitANGPT2 适用: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed only at sites of vascular remodeling.
- 板类型
- Pre-coated
- 实验流程
- mouse Angiopoietin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(CHO, M1-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Angiopoietin-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.15, Standard deviation: 0.044, CV(%): 3.8
- Sample 2: n=16, Mean(ng/ml): 3.53, Standard deviation: 0.148, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 7.21, Standard deviation: 0.368, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 1.18, Standard deviation: 0.103, CV(%): 8.7
- Sample 2: n=24, Mean(ng/ml): 3.62, Standard deviation: 0.308, CV(%): 8.5
- Sample 3: n=24, Mean(ng/ml): 7.34, Standard deviation: 0.558, CV(%): 7.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Angiopoietin 2 (ANGPT2) ELISA试剂盒
- Angiopoietin 2 (ANGPT2)
- 别名
- ANGPT2 (ANGPT2 产品)
- 别名
- AGPT2 ELISA Kit, ANG2 ELISA Kit, Agpt2 ELISA Kit, Ang-2 ELISA Kit, Ang2 ELISA Kit, ang2 ELISA Kit, angpt2 ELISA Kit, ANGIOPOIETIN-2 ELISA Kit, ANG-2 ELISA Kit, ANGPT2 ELISA Kit, Angrp ELISA Kit, Rnase5b ELISA Kit, ANG3 ELISA Kit, C11orf2 ELISA Kit, C11orf3 ELISA Kit, FFR ELISA Kit, angiopoietin 2 ELISA Kit, angiopoietin 2b ELISA Kit, angiogenin, ribonuclease A family, member 2 ELISA Kit, VPS51, GARP complex subunit ELISA Kit, ANGPT2 ELISA Kit, Angpt2 ELISA Kit, angpt2b ELISA Kit, Ang2 ELISA Kit, VPS51 ELISA Kit
- 背景
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Protein Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). .
Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Synonyms: Angiopoietin-2,ANG-2,Angpt2,Agpt2,
Full Gene Name: Angiopoietin-2
Cellular Localisation: Secreted. - 基因ID
- 11601
- UniProt
- O35608
- 途径
- RTK signaling
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