Clusterin ELISA 试剂盒
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- 抗原 See all Clusterin (CLU) ELISA试剂盒
- Clusterin (CLU)
- 抗原表位
- AA 22-226, AA 227-448
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Clusterin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: D22-R226(beta)&S227-E448(alpha) - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: D22-R226(beta)&S227-E448(alpha) - Featured
- Discover our best selling CLU ELISA Kit
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- Discover our top product CLU ELISA Kit
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Clusterin (CLU) ELISA Kit
CLU 适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Clusterin (CLU) ELISA KitCLU 适用: 小鼠 Colorimetric Sandwich ELISA 18.8 ng/mL - 1200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
Clusterin (CLU) ELISA KitCLU 适用: 小鼠 Colorimetric Sandwich ELISA 2.5 ng/mL - 160 ng/mL Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: 马 Colorimetric Competition ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Clusterin (CLU) ELISA KitCLU 适用: 人 AA 23-227 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Most abundant in stomach, liver, brain, and testis, with intermediate levels in heart, ovary and kidney.
- 板类型
- Pre-coated
- 实验流程
- mouse Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D22-R226(β)&S227-E448(α)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Clusterin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Clusterin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.086, CV(%): 6.5
- Sample 2: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.275, CV(%): 4.1
- Sample 3: n=16, Mean(ng/ml): 13.5, Standard deviation: 0.567, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 1.43, Standard deviation: 0.12, CV(%): 8.4
- Sample 2: n=24, Mean(ng/ml): 7.4, Standard deviation: 0.555, CV(%): 7.5
- Sample 3: n=24, Mean(ng/ml): 14.1, Standard deviation: 1.114, CV(%): 7.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Clusterin (CLU) ELISA试剂盒
- Clusterin (CLU)
- 别名
- CLU (CLU 产品)
- 别名
- LOC100136120 ELISA Kit, clu ELISA Kit, MGC53055 ELISA Kit, zgc:73352 ELISA Kit, xclu ELISA Kit, MGC89163 ELISA Kit, CLU ELISA Kit, APO-J ELISA Kit, APOJ ELISA Kit, CLI ELISA Kit, CLU1 ELISA Kit, CLU2 ELISA Kit, KUB1 ELISA Kit, NA1/NA2 ELISA Kit, SGP-2 ELISA Kit, SGP2 ELISA Kit, SP-40 ELISA Kit, TRPM-2 ELISA Kit, TRPM2 ELISA Kit, DAG ELISA Kit, RATTRPM2B ELISA Kit, SP40 ELISA Kit, TRPM2B ELISA Kit, Trpm2 ELISA Kit, Trpmb ELISA Kit, AI893575 ELISA Kit, ApoJ ELISA Kit, Cli ELISA Kit, D14Ucla3 ELISA Kit, Sgp-2 ELISA Kit, Sgp2 ELISA Kit, Sugp-2 ELISA Kit, GP80 ELISA Kit, gpIII ELISA Kit, 40 ELISA Kit, clusterin-1 ELISA Kit, clusterin L homeolog ELISA Kit, clusterin ELISA Kit, Clusterin ELISA Kit, LOC100136120 ELISA Kit, clu.L ELISA Kit, clu ELISA Kit, CLU ELISA Kit, clus ELISA Kit, Clu ELISA Kit, LOC101113728 ELISA Kit
- 背景
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Protein Function: Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation (By similarity). .
Background: Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several Synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
Synonyms: Clusterin,Apolipoprotein J,Apo-J,Clustrin,Sulfated glycoprotein 2,SGP-2,Clusterin beta chain,Clusterin alpha chain,Clu,Apoj, Msgp-2,
Full Gene Name: Clusterin
Cellular Localisation: Secreted . Nucleus . Cytoplasm . Mitochondrion membrane, Peripheral membrane protein, Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis (By similarity).. - 基因ID
- 12759
- UniProt
- Q06890
- 途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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