SCGB1A1 ELISA 试剂盒
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- 抗原 See all SCGB1A1 ELISA试剂盒
- SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))
- 抗原表位
- AA 22-91
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SCGB1A1/uteroglobin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: E22-N91 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: E22-N91 - Featured
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- Discover our top product SCGB1A1 ELISA Kit
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Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA Kit
SCGB1A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSmall Sample SCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
- 板类型
- Pre-coated
- 实验流程
- human SCGB1A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1 has been precoated onto 96-well plates. Standards(E.coli, E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCGB1A1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human SCGB1A1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human SCGB1A1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.2, Standard deviation: 0.071, CV(%): 5.9
- Sample 2: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.085, CV(%): 3.7
- Sample 3: n=16, Mean(ng/ml): 5.2, Standard deviation: 0.276, CV(%): 5.3,
- Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.101, CV(%): 6.3
- Sample 2: n=24, Mean(ng/ml): 2.8, Standard deviation: 0.154, CV(%): 5.5
- Sample 3: n=24, Mean(ng/ml): 6.1, Standard deviation: 0.464, CV(%): 7.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all SCGB1A1 ELISA试剂盒
- SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))
- 别名
- SCGB1A1 (SCGB1A1 产品)
- 别名
- CC10 ELISA Kit, CC16 ELISA Kit, CCPBP ELISA Kit, CCSP ELISA Kit, UGB ELISA Kit, UP-1 ELISA Kit, UP1 ELISA Kit, UGL ELISA Kit, PCB-BP ELISA Kit, UG ELISA Kit, Utg ELISA Kit, PCBB ELISA Kit, Ugb ELISA Kit, SCGB1A1 ELISA Kit, secretoglobin family 1A member 1 ELISA Kit, secretoglobin, family 1A, member 1 (uteroglobin) ELISA Kit, urinary protein 2 ELISA Kit, SCGB1A1 ELISA Kit, Scgb1a1 ELISA Kit, Rup2 ELISA Kit
- 背景
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Protein Function: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Background: SCGB1A1(secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.
Synonyms: Uteroglobin,Clara cell phospholipid-binding protein,CCPBP,Clara cells 10 kDa secretory protein,CC10,Secretoglobin family 1A member 1,Urinary protein 1,UP-1,UP1,Urine protein 1,SCGB1A1,CC10, CCSP, UGB,
Full Gene Name: Uteroglobin
Cellular Localisation: Secreted. - 基因ID
- 7356
- UniProt
- P11684
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