FURIN ELISA 试剂盒
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- 抗原 See all FURIN ELISA试剂盒
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
- 抗原表位
- AA 108-715
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Furin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Tissue Homogenate, Cell Lysate
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: D108-E715 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: D108-E715 - Featured
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Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA Kit
FURIN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S8 family. Furin subfamily.
Tissue Specificity: Seems to be expressed ubiquitously.
- 板类型
- Pre-coated
- 实验流程
- human Furin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Furin has been precoated onto 96-well plates. Standards(NSO, D108-E715) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Furin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Furin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Furin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.9, Standard deviation: 0.091, CV(%): 4.8
- Sample 2: n=16, Mean(ng/ml): 8.8, Standard deviation: 0.493, CV(%): 5.6
- Sample 3: n=16, Mean(ng/ml): 15.7, Standard deviation: 0.942, CV(%): 6,
- Sample 1: n=24, Mean(ng/ml): 2.3, Standard deviation: 0.122, CV(%): 5.3
- Sample 2: n=24, Mean(ng/ml): 9.2, Standard deviation: 0.589, CV(%): 6.4
- Sample 3: n=24, Mean(ng/ml): 17.4, Standard deviation: 1.27, CV(%): 7.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all FURIN ELISA试剂盒
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
- 别名
- FURIN (FURIN 产品)
- 别名
- FURIN ELISA Kit, furin ELISA Kit, si:dkey-281a24.5 ELISA Kit, FUR ELISA Kit, LOC100220259 ELISA Kit, PACE ELISA Kit, spc1 ELISA Kit, xfurin ELISA Kit, MGC82710 ELISA Kit, PCSK3 ELISA Kit, SPC1 ELISA Kit, 9130404I01Rik ELISA Kit, Fur ELISA Kit, Pcsk3 ELISA Kit, Pace ELISA Kit, furin, paired basic amino acid cleaving enzyme ELISA Kit, furin (paired basic amino acid cleaving enzyme) a ELISA Kit, furin (paired basic amino acid cleaving enzyme) b ELISA Kit, furin, paired basic amino acid cleaving enzyme L homeolog ELISA Kit, furin (paired basic amino acid cleaving enzyme) ELISA Kit, FURIN ELISA Kit, furina ELISA Kit, furinb ELISA Kit, furin ELISA Kit, furin.L ELISA Kit, Furin ELISA Kit
- 背景
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Protein Function: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. .
Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance
Synonyms: Furin,3.4.21.75,Dibasic-processing enzyme,Paired basic amino acid residue-cleaving enzyme,PACE,FURIN,FUR, PACE, PCSK3,
Full Gene Name: Furin
Cellular Localisation: Golgi apparatus, trans-Golgi network membrane, Single-pass type I membrane protein. Cell membrane, Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - 基因ID
- 5045
- UniProt
- P09958
- 途径
- Notch Signaling, Neurotrophin Signaling Pathway
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