抗原 See all CCL2 ELISA试剂盒: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
抗原表位: AA 24-148
适用 20 16 9 4 4 4 4 3 3 2 2 2 1 1: 大鼠
检测方法: Colorimetric
实验类型: Sandwich ELISA
检测范围: 15.6-1000 pg/mL
最低检测浓度: 15.6 pg/mL
应用范围: ELISA
原理: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MCP-1/CCL2
品牌: PicoKine™
样品类型: Cell Culture Supernatant, Serum
Analytical Method: Quantitative
特异性: Expression system for standard: NSO
Immunogen sequence: Q24-N148
交叉反应（详细）: There is no detectable cross-reactivity with other relevant proteins.
灵敏度: <10pg/mL
试剂未包括: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
免疫原: Expression system for standard: NSO
Immunogen sequence: Q24-N148
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Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit

CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

rat MCP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCP-1 has been precoated onto 96-well plates. Standards(NSO, Q24-N148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MCP-1 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat MCP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MCP-1 standard solution and each sample be measured in duplicate.

实验精密度

Sample 1: n=16, Mean(pg/ml): 87, Standard deviation: 3.306, CV(%): 3.8
Sample 2: n=16, Mean(pg/ml): 318, Standard deviation: 16.54, CV(%): 5.2
Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 36.4, CV(%): 5.8,
Sample 1: n=24, Mean(pg/ml): 98, Standard deviation: 4.802, CV(%): 4.9
Sample 2: n=24, Mean(pg/ml): 346, Standard deviation: 18.34, CV(%): 5.3
Sample 3: n=24, Mean(pg/ml): 683, Standard deviation: 42.35, CV(%): 6.2

限制

仅限研究用

注意事项: Avoid multiple freeze-thaw cycles.
储存条件: -20 °C,4 °C
储存方法: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
有效期: 12 months

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抗原 See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

别名

CCL2 (CCL2 产品)

别名

GDCF-2 ELISA Kit, HC11 ELISA Kit, HSMCR30 ELISA Kit, MCAF ELISA Kit, MCP-1 ELISA Kit, MCP1 ELISA Kit, SCYA2 ELISA Kit, SMC-CF ELISA Kit, AI323594 ELISA Kit, JE ELISA Kit, Scya2 ELISA Kit, Sigje ELISA Kit, MCP-1A ELISA Kit, MCP1A ELISA Kit, C-C motif chemokine ligand 2 ELISA Kit, chemokine (C-C motif) ligand 2 ELISA Kit, C-C motif chemokine 2 ELISA Kit, CCL2 ELISA Kit, Ccl2 ELISA Kit, LOC101120093 ELISA Kit

背景

Protein Function: Chemotactic factor that attracts monocytes, but not neutrophils.

Background: Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

Synonyms: C-C motif chemokine 2,Immediate-early serum-responsive protein JE,Monocyte chemoattractant protein 1,Monocyte chemotactic protein 1,MCP-1,Small-inducible cytokine A2,Ccl2,Je, Mcp1, Scya2,

Full Gene Name: C-C motif chemokine 2

Cellular Localisation: Secreted.

基因ID

24770

UniProt

P14844

途径

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection

CCL2 ELISA 试剂盒

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贮存及处理

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使用细节

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