BMP7 ELISA 试剂盒
(5 references)-
- 抗原 See all BMP7 ELISA试剂盒
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- 抗原表位
- AA 293-431
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-7
- 品牌
- PicoKine™
- 样品类型
- Osseous Tissue, Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: S293-H431 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: S293-H431 - Featured
- Discover our best selling BMP7 ELISA Kit
- Top Product
- Discover our top product BMP7 ELISA Kit
-
Bone Morphogenetic Protein 7 (BMP7) ELISA Kit
BMP7 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 绵羊 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Hamster AA 292-430 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 小鼠 AA 292-430 Colorimetric Sandwich ELISA 31.2-2000 pg/mL
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 AA 292-430 Colorimetric Sandwich ELISA 31.2-2000 pg/mL
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Bone, Cell Culture Supernatant, Plasma, Serum, Urine
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(NSO, S293-H431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-7 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-7 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 19.8, CV(%): 5.8
- Sample 2: n=16, Mean(pg/ml): 765, Standard deviation: 42.8, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 1108, Standard deviation: 57.6, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 372, Standard deviation: 30.5, CV(%): 8.2
- Sample 2: n=24, Mean(pg/ml): 780, Standard deviation: 62.4, CV(%): 8
- Sample 3: n=24, Mean(pg/ml): 1265, Standard deviation: 98.7, CV(%): 7.8
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." in: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).
: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." in: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).
: "Changes in the expression of bone morphogenetic protein 7 and tamm- horsfall protein in the early stages of diabetic nephropathy." in: Nephro-urology monthly, Vol. 4, Issue 2, pp. 466-9, (2013) (PubMed).
: "Effects of Panax notoginoside on the nephropathy in rats with type 1 diabetes mellitus." in: Chinese journal of integrative medicine, Vol. 17, Issue 8, pp. 612-5, (2011) (PubMed).
: "Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 18, Issue 4, pp. 496-503, (2010) (PubMed).
-
: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." in: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).
-
- 抗原 See all BMP7 ELISA试剂盒
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- 别名
- BMP7 (BMP7 产品)
- 别名
- bmp-7 ELISA Kit, bmp7 ELISA Kit, op-1 ELISA Kit, BMP7 ELISA Kit, zgc:153025 ELISA Kit, OP-1 ELISA Kit, OP1 ELISA Kit, BMP-7 ELISA Kit, fb83f09 ELISA Kit, wu:fb83f09 ELISA Kit, bone morphogenetic protein 7 ELISA Kit, bone morphogenetic protein 7, gene 1 L homeolog ELISA Kit, bone morphogenetic protein 7b ELISA Kit, bone morphogenetic protein 7, gene 2 L homeolog ELISA Kit, bone morphogenetic protein 7a ELISA Kit, BMP7 ELISA Kit, bmp7.1.L ELISA Kit, LOC100136282 ELISA Kit, bmp7b ELISA Kit, Bmp7 ELISA Kit, LOC100008826 ELISA Kit, bmp7.2.L ELISA Kit, bmp7a ELISA Kit
- 背景
-
Background: Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
Synonyms: Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ,cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ,cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1)(BMP7), mRNA ,BMP7 ,hCG_40100 ,
Full Gene Name: bone morphogenetic protein 7
- 基因ID
- 655
- UniProt
- A8K571
- 途径
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
-
