PTX3 ELISA 试剂盒
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- 抗原 See all PTX3 ELISA试剂盒
- PTX3 (Pentraxin 3 (PTX3))
- 抗原表位
- AA 18-381
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PTX3/Pentraxin 3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: E18-S381 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: E18-S381 - Featured
- Discover our best selling PTX3 ELISA Kit
- Top Product
- Discover our top product PTX3 ELISA Kit
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Pentraxin 3 (PTX3) ELISA Kit
PTX3 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 人 AA 18-381 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Saliva, Serum
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: Cow Colorimetric Sandwich ELISA 2.344-150 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PTX3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse PTX3 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1274, Standard deviation: 56.1, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 2937, Standard deviation: 164.5, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 5436, Standard deviation: 342.5, CV(%): 6.3,
- Sample 1: n=24, Mean(pg/ml): 1435, Standard deviation: 83.23, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 3356, Standard deviation: 245, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 5628, Standard deviation: 382.7, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all PTX3 ELISA试剂盒
- PTX3 (Pentraxin 3 (PTX3))
- 别名
- PTX3 (PTX3 产品)
- 别名
- fb05a06 ELISA Kit, wu:fb05a06 ELISA Kit, TNFAIP5 ELISA Kit, TSG-14 ELISA Kit, PPTX ELISA Kit, AI607804 ELISA Kit, si:ch211-272f3.3 ELISA Kit, pentraxin 3 ELISA Kit, pentraxin related gene ELISA Kit, si:ch211-272f3.3 ELISA Kit, LOC100230343 ELISA Kit, PTX3 ELISA Kit, Ptx3 ELISA Kit
- 背景
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Protein Function: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .
Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.
Synonyms: Pentraxin-related protein PTX3,Pentaxin-related protein PTX3,Tumor necrosis factor-inducible gene 14 protein,TSG-14,Ptx3,Tsg14,
Full Gene Name: Pentraxin-related protein PTX3
Cellular Localisation: Secreted. - 基因ID
- 19288
- UniProt
- P48759
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