CTLA4 ELISA 试剂盒
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- 抗原 See all CTLA4 ELISA试剂盒
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 抗原表位
- AA 37-162
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CTLA4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A37-F162 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A37-F162 - Featured
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- Discover our top product CTLA4 ELISA Kit
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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA Kit
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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 93.75-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 5-1200 pg/mL Cell Culture Supernatant, Plasma, Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Widely expressed with highest levels in lymphoid tissues. .
- 板类型
- Pre-coated
- 实验流程
- mouse CTLA4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CTLA4 has been precoated onto 96-well plates. Standards(NSO, A37-F162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CTLA4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CTLA4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CTLA4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CTLA4 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.42, Standard deviation: 0.051, CV(%): 3.6
- Sample 2: n=16, Mean(ng/ml): 3.53, Standard deviation: 0.138, CV(%): 3.9
- Sample 3: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.286, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.063, CV(%): 4.3
- Sample 2: n=24, Mean(ng/ml): 3.71, Standard deviation: 0.171, CV(%): 4.6
- Sample 3: n=24, Mean(ng/ml): 6.87, Standard deviation: 0.426, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CTLA4 ELISA试剂盒
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 别名
- CTLA4 (CTLA4 产品)
- 别名
- CTLA-4 ELISA Kit, CTLA4 ELISA Kit, CD152 ELISA Kit, Cd152 ELISA Kit, Ctla-4 ELISA Kit, Ly-56 ELISA Kit, CD ELISA Kit, CELIAC3 ELISA Kit, GRD4 ELISA Kit, GSE ELISA Kit, IDDM12 ELISA Kit, sCTLA4 ELISA Kit, cytotoxic T-lymphocyte associated protein 4 ELISA Kit, cytotoxic T-lymphocyte-associated protein 4 ELISA Kit, CTLA4 ELISA Kit, Ctla4 ELISA Kit
- 背景
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Protein Function: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity). .
Background: CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
Synonyms: Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4,CTLA-4,CD152,Ctla4,Cd152,
Full Gene Name: Cytotoxic T-lymphocyte protein 4
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 12477
- UniProt
- P09793
- 途径
- Cancer Immune Checkpoints
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