CD163 ELISA 试剂盒
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- 抗原 See all CD163 ELISA试剂盒
- CD163
- 抗原表位
- AA 41-1045
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56-100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD163
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: G41-S1045 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <150pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: G41-S1045 - Featured
- Discover our best selling CD163 ELISA Kit
- Top Product
- Discover our top product CD163 ELISA Kit
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CD163 (CD163) ELISA Kit
CD163 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
CD163 (CD163) ELISA KitCD163 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
CD163 (CD163) ELISA KitCD163 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
CD163 (CD163) ELISA KitCD163 适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 猴 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
CD163 (CD163) ELISA KitCD163 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: Pig Colorimetric Sandwich ELISA 93.75 ng/mL - 6000 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 9 SRCR domains.
Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. .
- 板类型
- Pre-coated
- 实验流程
- human CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD163 has been precoated onto 96-well plates. Standards(NSO, G41-S1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD163 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL,50 ng/mL, 25 ng/mL,12.5 ng/mL,6.25 ng/mL,3.13 ng/mL,1.56 ng/mL human CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD163 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 24, Standard deviation: 1.008, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 38.1, Standard deviation: 1.715, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 65, Standard deviation: 3.445, CV(%): 5.3,
- Sample 1: n=24, Mean(ng/ml): 23, Standard deviation: 1.541, CV(%): 6.7
- Sample 2: n=24, Mean(ng/ml): 37.2, Standard deviation: 2.009, CV(%): 5.4
- Sample 3: n=24, Mean(ng/ml): 67, Standard deviation: 3.752, CV(%): 5.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CD163 ELISA试剂盒
- CD163
- 别名
- CD163 (CD163 产品)
- 别名
- DMBT1 ELISA Kit, CD163 ELISA Kit, CD163v2 ELISA Kit, CD163v3 ELISA Kit, M130 ELISA Kit, MM130 ELISA Kit, deleted in malignant brain tumors 1 protein-like ELISA Kit, CD163 molecule ELISA Kit, CD163 antigen ELISA Kit, LOC426820 ELISA Kit, CD163 ELISA Kit, Cd163 ELISA Kit
- 背景
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Protein Function: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH -dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
Synonyms: Scavenger receptor cysteine-rich type 1 protein M130,Hemoglobin scavenger receptor,CD163,Soluble CD163,sCD163,CD163,M130,
Full Gene Name: Scavenger receptor cysteine-rich type 1 protein M130
Cellular Localisation: Soluble CD163: Secreted. - 基因ID
- 9332
- UniProt
- Q86VB7
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