CD36 ELISA 试剂盒
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- 抗原 See all CD36 ELISA试剂盒
- CD36
- 抗原表位
- AA 30-439
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD36/SR-B3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: G30-K439 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: G30-K439 - Featured
- Discover our best selling CD36 ELISA Kit
- Top Product
- Discover our top product CD36 ELISA Kit
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CD36 (CD36) ELISA Kit
CD36 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
CD36 (CD36) ELISA KitCD36 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 人 AA 30-439 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD36 (CD36) ELISA KitCD36 适用: 大鼠 AA 30-439 Colorimetric Sandwich ELISA 218-14.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD36 (CD36) ELISA KitCD36 适用: 小鼠 Colorimetric Sandwich ELISA 30-20000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD36 (CD36) ELISA KitCD36 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD36 (CD36) ELISA KitCD36 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD36 has been precoated onto 96-well plates. Standards(CHO, G30-K439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD36 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD36 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD36 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.24, Standard deviation: 0.052, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 3.31, Standard deviation: 0.235, CV(%): 7.1
- Sample 3: n=16, Mean(ng/ml): 5.28, Standard deviation: 0.391, CV(%): 7.4,
- Sample 1: n=24, Mean(ng/ml): 1.36, Standard deviation: 0.072, CV(%): 5.3
- Sample 2: n=24, Mean(ng/ml): 4.16, Standard deviation: 0.349, CV(%): 8.4
- Sample 3: n=24, Mean(ng/ml): 6.24, Standard deviation: 0.530, CV(%): 8.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CD36 ELISA试剂盒
- CD36
- 别名
- CD36 (CD36 产品)
- 别名
- BDPLT10 ELISA Kit, CHDS7 ELISA Kit, FAT ELISA Kit, GP3B ELISA Kit, GP4 ELISA Kit, GPIV ELISA Kit, PASIV ELISA Kit, SCARB3 ELISA Kit, Fat ELISA Kit, Scarb3 ELISA Kit, GPIIIB ELISA Kit, PAS-4 ELISA Kit, zgc:92513 ELISA Kit, CD36 molecule ELISA Kit, CD36 molecule (thrombospondin receptor) ELISA Kit, CD36 ELISA Kit, Cd36 ELISA Kit, cd36 ELISA Kit
- 背景
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Protein Function: Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .
Background: CD36(Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV(gpIV) and glycoprotein IIIb(gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Synonyms: Platelet glycoprotein 4,Glycoprotein IIIb,GPIIIB,PAS IV,PAS-4,Platelet glycoprotein IV,GPIV,CD36,Cd36,
Full Gene Name: Platelet glycoprotein 4
Cellular Localisation: Cell membrane, Multi-pass membrane protein. Upon ligand-binding, internalized through dynamin-dependent endocytosis.. - 基因ID
- 12491
- UniProt
- Q08857
- 途径
- TLR signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins
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