Prolactin ELISA 试剂盒
(3 references)-
- 抗原 See all Prolactin (PRL) ELISA试剂盒
- Prolactin (PRL)
- 抗原表位
- AA 29-227
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Prolactin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: L29-C227 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: L29-C227 - Top Product
- Discover our top product PRL ELISA Kit
-
Prolactin (PRL) ELISA Kit
PRL 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 犬 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 绵羊 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 山羊 Colorimetric Competition ELISA 31.25 IU/mL - 2000 IU/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 小鸡 Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 兔 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the somatotropin/prolactin family.
- 板类型
- Pre-coated
- 实验流程
- human Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Prolactin has been precoated onto 96-well plates. Standards(E.coli, L29-C227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Prolactin amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Prolactin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Prolactin standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1491, Standard deviation: 85, CV(%): 5.7
- Sample 2: n=16, Mean(pg/ml): 2734, Standard deviation: 134, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 4962, Standard deviation: 248.1, CV(%): 5,
- Sample 1: n=24, Mean(pg/ml): 1529, Standard deviation: 96.33, CV(%): 6.3
- Sample 2: n=24, Mean(pg/ml): 2850, Standard deviation: 196.7, CV(%): 6.9
- Sample 3: n=24, Mean(pg/ml): 5271, Standard deviation: 390, CV(%): 7.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 40, pp. E8468-E8477, (2018) (PubMed).
: "Chronic endometritis modifies decidualization in human endometrial stromal cells." in: Reproductive biology and endocrinology : RB&E, Vol. 15, Issue 1, pp. 16, (2017) (PubMed).
: "Influenza A(H1N1) vaccination during early pregnancy transiently promotes hippocampal neurogenesis and working memory. Involvement of Th1/Th2 balance." in: Brain research, Vol. 1592, pp. 34-43, (2015) (PubMed).
-
: "Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 40, pp. E8468-E8477, (2018) (PubMed).
-
- 抗原 See all Prolactin (PRL) ELISA试剂盒
- Prolactin (PRL)
- 别名
- PRL (PRL 产品)
- 背景
-
Protein Function: Prolactin acts primarily on the mammary gland by promoting lactation.
Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.
Synonyms: Prolactin,PRL,PRL,
Full Gene Name: Prolactin
Cellular Localisation: Secreted. - 基因ID
- 5617
- UniProt
- P01236
- 途径
- JAK/STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
-
