CCL4 ELISA 试剂盒
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- 抗原 See all CCL4 ELISA试剂盒
- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
- 抗原表位
- AA 24-92
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8-500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL4/MIP-1 beta
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A24-N92 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A24-N92 - Featured
- Discover our best selling CCL4 ELISA Kit
- Top Product
- Discover our top product CCL4 ELISA Kit
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Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA Kit
CCL4 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 小鸡 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 大鼠 AA 24-92 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: Pig Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Chemokine (C-C Motif) Ligand 4 (CCL4) ELISA KitCCL4 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
- 板类型
- Pre-coated
- 实验流程
- human CCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL4 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 32, Standard deviation: 1.3, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 91, Standard deviation: 3.8, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 324, Standard deviation: 15.2, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 33, Standard deviation: 2.1, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 87, Standard deviation: 5.8, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 319, Standard deviation: 24.9, CV(%): 7.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CCL4 ELISA试剂盒
- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
- 别名
- CCL4 (CCL4 产品)
- 别名
- ACT2 ELISA Kit, AT744.1 ELISA Kit, G-26 ELISA Kit, HC21 ELISA Kit, LAG-1 ELISA Kit, LAG1 ELISA Kit, MIP-1-beta ELISA Kit, MIP1B ELISA Kit, MIP1B1 ELISA Kit, SCYA2 ELISA Kit, SCYA4 ELISA Kit, CCL4 ELISA Kit, K203 ELISA Kit, Mip1-b ELISA Kit, Scya4 ELISA Kit, MIP-1beta ELISA Kit, MIP-1BETA ELISA Kit, MIP1b ELISA Kit, SCY4A ELISA Kit, Act-2 ELISA Kit, MIP-1B ELISA Kit, Mip1b ELISA Kit, ACT-2 ELISA Kit, AT744.2 ELISA Kit, CCL4L ELISA Kit, SCYA4L ELISA Kit, SCYA4L1 ELISA Kit, SCYA4L2 ELISA Kit, C-C motif chemokine ligand 4 ELISA Kit, chemokine (C-C motif) ligand 4 ELISA Kit, chemokine (C-C motif) ligand 4-like 1 ELISA Kit, C-C motif chemokine ligand 4 like 1 ELISA Kit, CCL4 ELISA Kit, Ccl4 ELISA Kit, CCL4L1 ELISA Kit
- 背景
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Protein Function: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. .
Background: Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
Synonyms: C-C motif chemokine 4,G-26 T-lymphocyte-secreted protein,HC21,Lymphocyte activation gene 1 protein,LAG-1,MIP-1-beta(1-69),Macrophage inflammatory protein 1-beta,MIP-1-beta,PAT 744,Protein H400,SIS-gamma,Small-inducible cytokine A4,T-cell activation protein 2,ACT-2,MIP-1-beta(3-69),CCL4,LAG1, MIP1B, SCYA4,
Full Gene Name: C-C motif chemokine 4
Cellular Localisation: Secreted. - 基因ID
- 6351
- UniProt
- P13236
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