Leptin Receptor ELISA 试剂盒
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- 抗原 See all Leptin Receptor (LEPR) ELISA试剂盒
- Leptin Receptor (LEPR)
- 抗原表位
- AA 22-839
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Leptin receptor
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: L22-G839 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <5pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: L22-G839 - Featured
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Leptin Receptor (LEPR) ELISA Kit
LEPR 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 绵羊 Soluble Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Leptin Receptor (LEPR) ELISA KitLEPR 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the type I cytokine receptor family. Type 2 subfamily.
Tissue Specificity: Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.
- 板类型
- Pre-coated
- 实验流程
- mouse Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin receptor has been precoated onto 96-well plates. Standards(NSO, L22-G839) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin receptor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin receptor amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1328, Standard deviation: 87.65, CV(%): 6.6
- Sample 2: n=16, Mean(pg/ml): 3836, Standard deviation: 168.8, CV(%): 4.4
- Sample 3: n=16, Mean(pg/ml): 6429, Standard deviation: 327.9, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 1656, Standard deviation: 125.9, CV(%): 7.6
- Sample 2: n=24, Mean(pg/ml): 4025, Standard deviation: 221.4, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 6872, Standard deviation: 474.2, CV(%): 6.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Leptin Receptor (LEPR) ELISA试剂盒
- Leptin Receptor (LEPR)
- 别名
- LEPR (LEPR 产品)
- 别名
- CD295 ELISA Kit, LEP-R ELISA Kit, LEPRD ELISA Kit, OB-R ELISA Kit, OBR ELISA Kit, obr ELISA Kit, cd295 ELISA Kit, ob-rb ELISA Kit, Fa ELISA Kit, LEPROT ELISA Kit, Leprb ELISA Kit, Modb1 ELISA Kit, OB-RGRP ELISA Kit, Obr ELISA Kit, db ELISA Kit, diabetes ELISA Kit, obese-like ELISA Kit, obl ELISA Kit, ObRb ELISA Kit, Ob-R ELISA Kit, Ob-RM ELISA Kit, lepr ELISA Kit, leptin receptor ELISA Kit, leptin receptor S homeolog ELISA Kit, LEPR ELISA Kit, lepr ELISA Kit, Lepr ELISA Kit, lepr.S ELISA Kit
- 背景
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Protein Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
Background: Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
Synonyms: Leptin receptor,LEP-R,B219,OB receptor,OB-R,CD295,Lepr,Db, Obr,
Full Gene Name: Leptin receptor
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 16847
- UniProt
- P48356
- 途径
- JAK/STAT Signaling, AMPK Signaling, Feeding Behaviour
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