GM-CSF ELISA 试剂盒
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- 抗原 See all GM-CSF (CSF2) ELISA试剂盒
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 抗原表位
- AA 18-144
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GM-CSF
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A18-E144 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A18-E144 - Featured
- Discover our best selling CSF2 ELISA Kit
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- Discover our top product CSF2 ELISA Kit
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kit
CSF2 适用: 小鼠 Colorimetric Sandwich ELISA 1.37 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 兔 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 AA 1-127 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 小鼠 AA 18-141 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the GM-CSF family.
- 板类型
- Pre-coated
- 实验流程
- human GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli, A18-E144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GM-CSF amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human GM-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GM-CSF standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 4.58, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 485, Standard deviation: 15.52, CV(%): 3.2
- Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 27.95, CV(%): 4.5,
- Sample 1: n=24, Mean(pg/ml): 107, Standard deviation: 6.53, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 463, Standard deviation: 29.63, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 35.23, CV(%): 5.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all GM-CSF (CSF2) ELISA试剂盒
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 别名
- CSF2 (CSF2 产品)
- 别名
- GMCSF ELISA Kit, GM-CSF ELISA Kit, CSF2 ELISA Kit, Csfgm ELISA Kit, Gm-CSf ELISA Kit, MGI-IGM ELISA Kit, Gm-csf ELISA Kit, Gmcsf ELISA Kit, CSF ELISA Kit, colony stimulating factor 2 ELISA Kit, colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit, CSF2 ELISA Kit, Csf2 ELISA Kit
- 背景
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Protein Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Background: Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 % nucleotide homology and 54 % amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
Synonyms: Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF,Molgramostin,Sargramostim,CSF2,GMCSF,
Full Gene Name: Granulocyte-macrophage colony-stimulating factor
Cellular Localisation: Secreted. - 基因ID
- 1437
- UniProt
- P04141
- 途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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