Intrinsic Factor ELISA 试剂盒
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- 抗原 See all Intrinsic Factor (GIF) ELISA试剂盒
- Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 3.125 ng/mL - 200 ng/mL
- 最低检测浓度
- 3.125 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GIF in human serum, plasma, tissue homogenates and other biological fluids.
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.95 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) ELISA Kit
GIF 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) ELISA KitGIF 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
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- 样本量
- 50 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately. - 实验流程
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This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all Intrinsic Factor (GIF) ELISA试剂盒
- Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))
- 别名
- GIF (GIF 产品)
- 别名
- GIF ELISA Kit, IF ELISA Kit, IFMH ELISA Kit, INF ELISA Kit, TCN3 ELISA Kit, AV073125 ELISA Kit, gastric intrinsic factor ELISA Kit, GIF ELISA Kit, Gif ELISA Kit
- 背景
- Alternative name: TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis
- 基因ID
- 2694
- UniProt
- P27352
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