Cathelicidin ELISA 试剂盒
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- 抗原 See all Cathelicidin (CAMP) ELISA试剂盒
- Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))
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适用
- Various Species
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.118 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
- Top Product
- Discover our top product CAMP ELISA Kit
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Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit
CAMP 适用: 人 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 大鼠 Colorimetric Competition ELISA 24.69 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 小鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.45-30 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Cathelicidin Antimicrobial Peptide (CAMP) ELISA KitCAMP 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 50 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately. - 实验流程
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This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all Cathelicidin (CAMP) ELISA试剂盒
- Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))
- 别名
- cAMP (CAMP 产品)
- 别名
- CAP-18 ELISA Kit, CAP18 ELISA Kit, CRAMP ELISA Kit, FALL-39 ELISA Kit, FALL39 ELISA Kit, LL37 ELISA Kit, CATH-2 ELISA Kit, Cnlp ELISA Kit, Cramp ELISA Kit, MCLP ELISA Kit, CATH-B1 ELISA Kit, CATHB1 ELISA Kit, CATHL2 ELISA Kit, CATHL7 ELISA Kit, CAP11 ELISA Kit, cathelicidin antimicrobial peptide ELISA Kit, CAMP ELISA Kit, LOC100136187 ELISA Kit, LOC100136439 ELISA Kit, Camp ELISA Kit
- 物质类
- Chemical
- 背景
- Alternative name: c-AMP, 3'-5'-Cyclic Adenosine Monophosphate, Adenosine Cyclophosphate
- 途径
- Cellular Response to Molecule of Bacterial Origin
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