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Immunoglobulin isotyping

IsoT 适用: 小鼠 Colorimetric Cell Culture Supernatant, Purified Protein Pre-coated
产品编号 ABIN1113186
发货至: 中国
  • 抗原 See all Ig 试剂盒
    Ig
    抗原表位
    Heavy & Light Chain
    适用
    • 4
    • 2
    • 1
    小鼠
    检测方法
    Colorimetric
    标记
    HRP
    应用范围
    Isotyping (IsoT)
    原理
    The method employs the quantitative sandwich enzyme immunoassay technique.
    品牌
    FastELISA®
    样品类型
    Cell Culture Supernatant, Purified Protein
    特异性
    Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,
    产品特性
    The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.
    Top Product
    Discover our top product Ig ELISA Kit
  • 说明

    One Step ELISA 30 min total time

    实验时间
    < 1 h
    板类型
    Pre-coated
    实验流程
    Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    试剂准备

    dilution

    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原
    Ig
    别名
    Immunoglobulins (Ig 产品)
    别名
    ATPIG Kit, ATPIQ Kit, A330005H02Rik Kit, AI315324 Kit, Ig Kit, ATPase phospholipid transporting 11C Kit, ATPase, class VI, type 11C Kit, ATP11C Kit, Atp11c Kit
    背景
    Isotyping of mouse immunoglobulins
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