OLR1 ELISA 试剂盒
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- 抗原 See all OLR1 ELISA试剂盒
- OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 灵敏度
- < 1 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Mouse LOX1 antibody 2. Lyophilized Mouse LOX1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse LOX1 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA Kit
OLR1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 兔 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 人 AA 61-273 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1) ELISA KitOLR1 适用: 小鼠 AA 60-363 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LOX1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LOX1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the LOX1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of LOX1 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 20 min at 2000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all OLR1 ELISA试剂盒
- OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
- 别名
- LOX1 (OLR1 产品)
- 别名
- OLR1 ELISA Kit, LOX1 ELISA Kit, Olr1 ELISA Kit, CLEC8A ELISA Kit, LOXIN ELISA Kit, SCARE1 ELISA Kit, SLOX1 ELISA Kit, LOX-1 ELISA Kit, SR-EI ELISA Kit, Scare1 ELISA Kit, Oldlr1 ELISA Kit, Oldr1 ELISA Kit, PLOX-1 ELISA Kit, oxidized low density lipoprotein receptor 1 ELISA Kit, oxidized low density lipoprotein (lectin-like) receptor 1 ELISA Kit, OLR1 ELISA Kit, Olr1 ELISA Kit
- 背景
- Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a 270-amino acid protein that in humans is encoded by the OLR1 gene, which spans approximately 15 kb and contains 6 exons. LOX1 is expressed in vascular-rich organs but not in lymphocytes, and it belongs to the C-type lectin superfamily. This protein binds, internalizes and degrades oxidized low-density lipoprotein. It may be involved in the regulation of Fas-induced apoptosis, and it may also play a role as a scavenger receptor. Mutations of the OLR1 gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease.
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