CD147 ELISA 试剂盒
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- 抗原 See all CD147 (BSG) ELISA试剂盒
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of Emmprin in tissue lysates or cell culture supernates.
- 样品类型
- Tissue Lysate, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 灵敏度
- < 2 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human Emmprin antibody 2. Lyophilized Human Emmprin standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Emmprin antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Basigin (Ok Blood Group) (BSG) ELISA Kit
BSG 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Lung Lavage Fluid, Plasma, Serum
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 AA 25-205 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human- Emmprin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Emmprin polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Emmprin amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Emmprin can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernate, tissue lysate or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge at 2-8°C for 15 min at 1000 x g within 30 min of collection. For eliminating the platelet effect, suggesting that further centrifugation for 10 min at 2-8°C at 10000 x g. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all CD147 (BSG) ELISA试剂盒
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
- 别名
- Emmprin / CD147 (BSG 产品)
- 别名
- EMMPRIN ELISA Kit, RPE7 ELISA Kit, 5F7 ELISA Kit, CD147 ELISA Kit, M6 ELISA Kit, OK ELISA Kit, TCSF ELISA Kit, AI115436 ELISA Kit, AI325119 ELISA Kit, HT-7 ELISA Kit, 5A11 ELISA Kit, CE9 ELISA Kit, HT7 ELISA Kit, Ox47R ELISA Kit, basignin ELISA Kit, wu:fb26a05 ELISA Kit, wu:fo98e12 ELISA Kit, bsg ELISA Kit, prso03 ELISA Kit, basigin (Ok blood group) ELISA Kit, basigin ELISA Kit, basigin (Ok blood group) S homeolog ELISA Kit, BSG ELISA Kit, Bsg ELISA Kit, bsg ELISA Kit, bsg.S ELISA Kit
- 背景
- Cluster of Differentiation 147 (CD147) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin (BSG), is a member of the immunoglobulin superfamily, It interacts with fibroblasts and stimulates expression of MMPs, and plays an important role in tumor invasiveness and metastasis. This protein is a determinant for the Ok blood group system, and it has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum.
- 途径
- S100 Proteins
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