CD40 ELISA 试剂盒
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- 抗原 See all CD40 ELISA试剂盒
- CD40
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of CD40 in Human serum, body fluids, tissue lysates or cell culture supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 1 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human CD40 antibody 2. Lyophilized Human CD40 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human CD40 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Discover our best selling CD40 ELISA Kit
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- Discover our top product CD40 ELISA Kit
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CD40 (CD40) ELISA Kit
CD40 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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CD40 (CD40) ELISA KitCD40 适用: 小鼠 Colorimetric Sandwich ELISA 3-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 50-40000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
CD40 (CD40) ELISA KitCD40 适用: 人 AA 21-193 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
CD40 (CD40) ELISA KitCD40 适用: 小鼠 AA 24-193 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
CD40 (CD40) ELISA KitCD40 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD40 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD40 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the CD40 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CD40 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C . For cell lysate, add lysis solution before centrifugation.
Tissue lysate or body fluids: Centrifuge at approximately 2000 × g for 20 min to remove precipitate.
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all CD40 ELISA试剂盒
- CD40
- 别名
- CD40 / TNFRSF5 (CD40 产品)
- 别名
- Bp50 ELISA Kit, CDW40 ELISA Kit, TNFRSF5 ELISA Kit, p50 ELISA Kit, AI326936 ELISA Kit, GP39 ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, T-BAM ELISA Kit, TRAP ELISA Kit, Tnfrsf5 ELISA Kit, TNFSF5 ELISA Kit, CD40 molecule ELISA Kit, CD40 antigen ELISA Kit, CD40 ELISA Kit, Cd40 ELISA Kit
- 背景
- CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. It is expressed on the surface of all mature B cells. This protein receptor is a member of the TNF-receptor superfamily. It has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
- 途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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