Cathepsin B ELISA 试剂盒
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- 抗原 See all Cathepsin B (CTSB) ELISA试剂盒
- Cathepsin B (CTSB)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of Cathepsin B in tissue lysates or cell culture supernates.
- 样品类型
- Tissue Lysate, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 灵敏度
- < 5 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Mouse Cathepsin B antibody 2. Lyophilized Mouse Cathepsin B standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse Cathepsin B antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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Cathepsin B (CTSB) ELISA Kit
CTSB 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 440 Cathepsin B Assay KitFRET Fluorometric
SensoLyte® 520 Cathepsin B Assay KitFRET Fluorometric
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cathepsin B (CTSB) ELISA KitCTSB 适用: 人 AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Cathepsin B polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Cathepsin B polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Cathepsin B amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Cathepsin B can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernate, tissue lysate or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin as the anticoagulant. Centrifuge at 2-8°C for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate and EDTA can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all Cathepsin B (CTSB) ELISA试剂盒
- Cathepsin B (CTSB)
- 别名
- Cathepsin B (CTSB 产品)
- 别名
- APPS ELISA Kit, CPSB ELISA Kit, CB ELISA Kit, apps ELISA Kit, cpsb ELISA Kit, LOC692390 ELISA Kit, CTSB ELISA Kit, cathepsin B ELISA Kit, cathepsin B S homeolog ELISA Kit, CTSB ELISA Kit, Ctsb ELISA Kit, ctsb.S ELISA Kit, LOC692390 ELISA Kit
- 背景
- Cathepsin B is an enzymatic protein belonging to the peptidase (or protease) families. In humans, it is coded by the CTSB gene. This protein is a lysosomal cysteine protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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