BMP5 ELISA 试剂盒
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- 抗原 See all BMP5 ELISA试剂盒
- BMP5 (Bone Morphogenetic Protein 5 (BMP5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of BMP-5 in human serum, plasma, body fluids, tissue lysates or cell culture supernates.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 10 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-human BMP-5 antibody 2. Lyophilized BMP-5 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human BMP-5 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
- Featured
- Discover our best selling BMP5 ELISA Kit
- Top Product
- Discover our top product BMP5 ELISA Kit
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Bone Morphogenetic Protein 5 (BMP5) ELISA Kit
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Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 人 AA 317-454 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Osseous Tissue, Serum
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 兔 AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 猴 AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: Pig AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 犬 AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: Cow AA 317-454 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 5 (BMP5) ELISA KitBMP5 适用: 人 Colorimetric Sandwich ELISA 0.25-60 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-5 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-5 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the BMP-5 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of BMP-5 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 10 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with EDTA, heparin, citrate as the anticoagulant. Centrifuge for 15 min at 2-8 at 1500 x g within 30 min of collection. For eliminating the platelet effect, suggesting that further centrifugation for 10 min at 2-8°C at 10000 x g. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all BMP5 ELISA试剂盒
- BMP5 (Bone Morphogenetic Protein 5 (BMP5))
- 别名
- BMP-5 (BMP5 产品)
- 别名
- bmp5l ELISA Kit, hm:zehl0669 ELISA Kit, zehl0669 ELISA Kit, zgc:64230 ELISA Kit, BMP5 ELISA Kit, AU023399 ELISA Kit, se ELISA Kit, bone morphogenetic protein 5 ELISA Kit, bmp5 ELISA Kit, BMP5 ELISA Kit, LOC100539495 ELISA Kit, Bmp5 ELISA Kit
- 背景
- Bone morphogenetic protein 5 (BMP-5) is a member of the transforming growth factor-beta superfamily of regulatory molecules. Bone morphogenetic proteins were originally identified by the ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site, and known for their ability to induce bone and cartilage development. BMP-5 is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. It may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. This protein also act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis.
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
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