Clostridium Difficile Toxin A and B ELISA 试剂盒

• 1x ELISA Plate 12x8 stripes
• 1 x 50 mL dilution buffer
• 1 x 2.0 mL Standard control Toxin A&B
• 1 x 7 mL conjugate
• 1 x 30 mL 10x washing buffer
• 1 x 14 mL substrate
• 1 x 7.5 stop solution with 0.5 M H2SO4
• 1x Manual

• Wash bottle
• Timer
• Paper towels or absorbent sheets
• Discard container
• Vortex mixer
• Microplate shaker
• Refrigerator
• Distilled water
• Microcentrifuge
• Microplate reader

• Sensitivity of the assay for TcdA: 0,5 ng/ml
• Sensitivity of the assay for TcdB: 1,0 ng/ml

Transfer about 50 µl liquid stool sample or take an equivalent amount (50 mg) of compact stool in 450 µl dilution buffer. Please only dilute the feces with the supplied dilution buffer, otherwise incorrect measurements can occur. Homogenize the suspension by suction and ejection from a disposable pipette or by vortexing. After leaving for a short time to allow sedimentation of stool particles the clarified supernatant can be used directly in the test. Automated equipment may be used with specimens that have been centrifuged 5 min by 2500 x g to remove any particulate matter.
Note: If overnight storage of the diluted sample is desirable, the storage should be done at -20 °C. Otherwise, in rare cases the test could lead to false positive results

• Wear gloves for all manipulations with potentially contaminated or toxic suspensions.
• All reagents must be at room temperature prior to their use in the assay.