8-OHDG ELISA 试剂盒
8-OHDG
适用: 人
Colorimetric
Competition ELISA
0.5-200 ng/mL
Urine
(88 references)-
- 抗原 See all 8-OHDG products
- 8-OHDG (8-Hydroxyguanosine (8-OHDG))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.5-200 ng/mL
- 最低检测浓度
- 0.5 ng/mL
- 应用范围
- ELISA
- 原理
- This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.
- 样品类型
- Urine
- Analytical Method
- Quantitative
- 特异性
- Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
- 产品特性
- For serum and tissue samples, Highly Sensitive 8-OHdG Check is recommended.
- 组件
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8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type)* 1 plate
Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial
Primary Antibody Solution 1 vial (6mL)
Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial
Secondary Antibody Solution: 1 vial (12mL)
Chromatic Solution: 3,3',5,5'-tetramethylbenzidine. 1 vial (0.25mL)
Diluting Solution: H2O2 containing buffer. 1 vial (12mL)
Washing Solution(5x): 2 vials (26mL x 2)
Reaction Terminating Solution: 1M Phosphoric acid. 1 vial
(12mL) Standard 8-OHdG Solution: Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL). 1 vial each
Plate Seal: 2 sheets - 试剂未包括
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Micropipet and chip (100 µL, 1000 µL)
Measuring pipet (10 mL, 20 mL)/ measuring cylinders
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet
Microplate reader (filter, 450 nm).
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- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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: "Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 5, pp. 655-7, (2013) (PubMed).
: "Renal podocyte injury in a rat model of type 2 diabetes is prevented by metformin." in: Experimental diabetes research, Vol. 2012, pp. 210821, (2012) (PubMed).
: "Association of base excision repair gene polymorphisms with ESRD risk in a Chinese population." in: Oxidative medicine and cellular longevity, Vol. 2012, pp. 928421, (2012) (PubMed).
: "The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke." in: The open neurology journal, Vol. 6, pp. 51-7, (2012) (PubMed).
: "Biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs) and DNA damage: a cross-sectional pilot study among roofers in South Florida." in: BMJ open, Vol. 2, Issue 4, (2012) (PubMed).
: "Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice." in: PLoS ONE, Vol. 7, Issue 3, pp. e33148, (2012) (PubMed).
: "Prevention of bleomycin-induced lung fibrosis in mice by a novel approach of parallel inhibition of cyclooxygenase and nitric-oxide donation using NCX 466, a prototype cyclooxygenase inhibitor and nitric-oxide donor." in: The Journal of pharmacology and experimental therapeutics, Vol. 341, Issue 2, pp. 493-9, (2012) (PubMed).
: "Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis." in: International journal of nephrology and renovascular disease, Vol. 5, pp. 9-14, (2012) (PubMed).
: "Deranged bioenergetics and defective redox capacity in T lymphocytes and neutrophils are related to cellular dysfunction and increased oxidative stress in patients with active systemic lupus erythematosus." in: Clinical & developmental immunology, Vol. 2012, pp. 548516, (2011) (PubMed).
: "Effect of Hachimijiogan against Renal Dysfunction and Involvement of Hypoxia-Inducible Factor-1α in the Remnant Kidney Model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 348686, (2011) (PubMed).
: "Renovascular protective effects of erythropoietin in patients with chronic kidney disease." in: Internal medicine (Tokyo, Japan), Vol. 50, Issue 18, pp. 1929-34, (2011) (PubMed).
: "Correlation of Ochratoxin A exposure to urinary levels of 8-hydroxydeoxyguanosine and malondialdehyde in a Turkish population." in: Bulletin of environmental contamination and toxicology, Vol. 86, Issue 3, pp. 258-62, (2011) (PubMed).
: "The evaluation of the oxidative stress parameters in patients with primary angle-closure glaucoma." in: PLoS ONE, Vol. 6, Issue 11, pp. e27218, (2011) (PubMed).
: "Increased response to oxidative stress challenge in Graves' ophthalmopathy orbital fibroblasts." in: Molecular vision, Vol. 17, pp. 2782-8, (2011) (PubMed).
: "Marijuana-induced mania in a healthy adolescent: a case report." in: General hospital psychiatry, Vol. 33, Issue 6, pp. 640.e3-4, (2011) (PubMed).
: "8-hydroxydeguanosine and nitrotyrosine are prognostic factors in urinary bladder carcinoma." in: International journal of clinical and experimental pathology, Vol. 4, Issue 3, pp. 267-75, (2011) (PubMed).
: "Changes in oxidative stress biomarker and gene expression levels in workers exposed to volatile organic compounds." in: Industrial health, Vol. 49, Issue 1, pp. 8-14, (2011) (PubMed).
: "Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 4, pp. 1249-60, (2010) (PubMed).
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: "Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study." in: Medical gas research, Vol. 2, Issue 1, pp. 27, (2013) (PubMed).
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- 抗原 See all 8-OHDG products
- 8-OHDG (8-Hydroxyguanosine (8-OHDG))
- Abstract
- 8-OHDG 产品
- 物质类
- Chemical
- 背景
- About 8-hydroxy-2'-deoxyguanosine (8-OHdG): 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals. This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum , tissue and cultured cells.
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